Anti-IGF1 antibody (Biotin) (ab83137)

Overview

  • Product nameAnti-IGF1 antibody (Biotin)
    See all IGF1 primary antibodies
  • Description
    Rabbit polyclonal to IGF1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human IGF1.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF1 (ab9572). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.
WB Use a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use a concentration of 0.25 - 0.1 µg/ml. Direct ELISA. Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.

Target

  • FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similaritiesBelongs to the insulin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names
    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

References for Anti-IGF1 antibody (Biotin) (ab83137)

ab83137 has not yet been referenced specifically in any publications.

Product Wall

Thank you for getting back to me. I had not as yet sent the information I have in regards to the antibodies and their use in ELISA as I was still waiting for some more, but I'll share what I have now.

The anti-IGF1 antibody (Biotin) (ab83137)...

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Thank you for getting back to me.

We do have one antibody which has been used in sandwich ELISA previously to detect human IGF1. This is antibody http://www.abcam.com/IGF1-antibody-Biotin-ab83137.htmland it is biotin conjugated. I am enquirin...

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Thank you for your message and for providing this further information.

To confirm, I can confirm this antibody has been tested and therefore guaranteed for human samples. The previous email stated that it has not been tested specifically wit...

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Thank you for your reply

Please note that theseantibodies have not been tested with with serum samples, and so I am sorry no pretreatments have been tested which we could provide you with information on. In theory the antibodiesshould work w...

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Thank you for your reply.

I would like to reassure you that ab83137 Anti-IGF1 antibody (Biotin) and ab9572 Anti-IGF1 antibody are tested and covered by our 6 month guarantee for sandwich ELISA together, and human samples. In the event that a...

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Further to my previous response, serum samples and other biological fluids are known to show an increase in background due to the many components found in these types of samples.

Do you have the readings for the standard and test samples as w...

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Thank you for your patience.

I try to help you as much as possible while my colleague is out of the office. I have read through all the e-mails related to your enquiry. In order to be able to identify the sources of the problem, it would be i...

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Thank you for your reply.

I can confirm that one vial of ab83137 Anti-IGF1 antibody (Biotin)contains 50 ug. This information is provided at the top of the online datasheet.

This particular antibodyis provided lyophilised. The data...

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Thank you for your reply.

Reviewing the details of the experiment you are planning, I can suggest to consider which antibodies you use. As you are using a secondary antibody in this case, the secondary antibody will pick up the captureantibo...

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Thank you once again for your enquiries.

To follow up from my previous email, I now have some further information regarding ab83137 Anti-IGF1 antibody (Biotin)which can be used in sandwich ELISA with ab9572.

ab83137 is the same an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"