Mouse monoclonal [24-60] to IGF1 Receptor alpha subunit
SpecificityWeakly reacts with Rabbit and Pig. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2 µg/mg of lysate. Substantially (>80%) inhibits IGF-1 binding.
Use a concentration of 1.5 µg/ml.
Application notesIs unsuitable for WB.
RelevanceIGF1 Receptor is a tetramer of 2 alpha and 2 beta chains, linked by disulfide bonds. IGF1 Receptor, like insulin receptor, is synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.
The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. It interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. IGF1 Receptor forms a hybrid receptor with INSR, the hybrid being a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R.
Cellular localizationMembrane; Single-pass type I membrane protein.
References for Anti-IGF1 Receptor alpha subunit antibody [24-60] (ab80548)
This product has been referenced in:
Middleton J et al. Insulin-like growth factor (IGF) receptor, IGF-I, interleukin-1 beta (IL-1 beta), and IL-6 mRNA expression in osteoarthritic and normal human cartilage. J Histochem Cytochem44:133-41 (1996).
Read more (PubMed: 8609369) »
Soos MA et al. Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes. Adv Exp Med Biol343:145-57 (1993).
Read more (PubMed: 7514333) »