Anti-IGF1 Receptor alpha subunit antibody [24-60] (ab80548)

Overview

  • Product nameAnti-IGF1 Receptor alpha subunit antibody [24-60]
    See all IGF1 Receptor alpha subunit primary antibodies
  • Description
    Mouse monoclonal [24-60] to IGF1 Receptor alpha subunit
  • SpecificityWeakly reacts with Rabbit and Pig. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IP, Inhibition Assaymore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Rabbit, Human, Pig
  • Immunogen

    IGF-1R/3T3 mouse fibroblasts transfected with human IGF1 Receptor alpha subunit cDNA.

  • Epitopeaa 184-283
  • Positive control
    • Placenta or breast carcinoma
  • General notes

    ab80548 acts as a weak IGF-like agonist in Bioassays (Thymidine incorporation into DNA).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesab80548 acts as a weak IGF-like agonist in Bioassays (Thymidine incorporation into DNA).
  • ClonalityMonoclonal
  • Clone number24-60
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2 µg/mg of lysate. Substantially (>80%) inhibits IGF-1 binding.
Inhibition Assay Use a concentration of 1.5 µg/ml.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceIGF1 Receptor is a tetramer of 2 alpha and 2 beta chains, linked by disulfide bonds. IGF1 Receptor, like insulin receptor, is synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. It interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. IGF1 Receptor forms a hybrid receptor with INSR, the hybrid being a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R.
    • Cellular localizationMembrane; Single-pass type I membrane protein.
    • Database links
    • Alternative names
      • CD221 antibody
      • CD221 antigen antibody
      • IGF I receptor antibody
      • IGFIR antibody
      • Insulin like growth factor I receptor antibody
      • JTK13 antibody
      see all

    References for Anti-IGF1 Receptor alpha subunit antibody [24-60] (ab80548)

    This product has been referenced in:
    • Middleton J  et al. Insulin-like growth factor (IGF) receptor, IGF-I, interleukin-1 beta (IL-1 beta), and IL-6 mRNA expression in osteoarthritic and normal human cartilage. J Histochem Cytochem 44:133-41 (1996). Read more (PubMed: 8609369) »
    • Soos MA  et al. Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes. Adv Exp Med Biol 343:145-57 (1993). Read more (PubMed: 7514333) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"