Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab10729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 130 , 95 kDa. As the receptor is very fragile and susceptible to damage during the cell lysis process a gentle lysis buffer should be used.|
|ELISA||Use a concentration of 0.5 - 1 µg/ml.|
|Neutralising||Use a concentration of 0.5 - 1.5 µg/ml. for a one-half maximal inhibition of cell surface IGFIsR mediated IGF on a responsive cell line.|
ab10729 has not yet been referenced specifically in any publications.