Anti-IGF1 Receptor beta subunit antibody [1-2] (ab80547)

Overview

  • Product nameAnti-IGF1 Receptor beta subunit antibody [1-2]
  • Description
    Mouse monoclonal [1-2] to IGF1 Receptor beta subunit
  • Tested applicationsSuitable for: WB, IP, Purificationmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis, Zebrafish, Goldfish, Orangutan
  • Immunogen

    Synthetic peptide:

    RKNERALPLPQSSTC

    , corresponding to C terminal amino acids 1323-1337 of Human IGF1 Receptor beta subunit

  • Epitopeaa 1323-1337
  • Positive control
    • Placenta or breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab80547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 95 kDa.
IP Use at an assay dependent dilution.
Purification Use at an assay dependent dilution.

Target

  • FunctionThis receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
  • Tissue specificityFound as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
  • Involvement in diseaseDefects in IGF1R are a cause of insulin-like growth factor 1 resistance (IGF1RES) [MIM:270450]. It is a disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 3 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
    Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD221 antibody
    • CD221 antigen antibody
    • EC 2.7.10.1 antibody
    • IGF I receptor antibody
    • IGF-I receptor antibody
    • Igf1r antibody
    • IGF1R_HUMAN antibody
    • IGFIR antibody
    • Insulin like growth factor 1 receptor alpha chain antibody
    • Insulin like growth factor 1 receptor beta chain antibody
    • Insulin like growth factor I receptor antibody
    • Insulin-like growth factor 1 receptor beta chain antibody
    • Insulin-like growth factor I receptor antibody
    • JTK13 antibody
    • MGC142170 antibody
    • MGC142172 antibody
    • MGC18216 antibody
    • Soluble IGF1R variant 1 antibody
    • Soluble IGF1R variant 2 antibody
    see all

References for Anti-IGF1 Receptor beta subunit antibody [1-2] (ab80547)

This product has been referenced in:
  • Soos MA  et al. Purified hybrid insulin/insulin-like growth factor-I receptors bind insulin-like growth factor-I, but not insulin, with high affinity. Biochem J 290 ( Pt 2):419-26 (1993). WB, AP, IP ; Human, Rat . Read more (PubMed: 8452530) »
  • Soos MA  et al. Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes. Adv Exp Med Biol 343:145-57 (1993). WB, AP, IP ; Human, Rat . Read more (PubMed: 7514333) »

See all 3 Publications for this product

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Thank you for sending your western blot protocol and the data. I am sorry to see that this antibody is not working for you in this application. Your protocol is correct and the blot with the other antibody demonstrates that the sample is not th...

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Thank you for contacting us and considering the participation in the testing discount scheme. These are the discount codes for the respective antibodies: ab63344 xxxxxxx ab80547 xxxxxxx Both are valid until the xth of xxx 20xx ...

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Thank you for contacting us. We do have one antibody that is specific for IGF1 receptor beta subunit as ab80547. http://www.abcam.com/IGF1-Receptor-beta-subunit-antibody-1-2-BSA-and-Azide-free-ab80547.html We have tested and would...

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