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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
Anti-IGF1 Receptor (phospho Y1161) antibody
See all IGF1 Receptor products (21) ...
Description
Rabbit polyclonal to IGF1 Receptor (phospho Y1161)
Specificity
Ab39398 detects endogenous levels of IGF1 Receptor only when phosphorylated at tyrosine 1161. The antibody cross-reacts with insulin receptor at phosphorylation site Y1185.
Tested applications
IHC-Fr, IHC-FoFr, ICC, IHC-P, WB, ELISA, IP, IHC-FrFl, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phospho-peptide derived from human IGF1 receptor around the phosphorylation site of tyrosine 1161
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab39398 was affinity purified from rabbit antiserum by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: 1/100(PubMed: 18216249)
ICC: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100.
WB: 1/50 - 1/100.Predicted molecular weight: 155 kDa.
ELISA: 1/20000
IP: Use at an assay dependent dilution.
IHC-FrFl: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution.
Target
Function
This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
Tissue specificity
Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
Involvement in disease
Defects in IGF1R are a cause of insulin-like growth factor 1 resistance (IGF1RES) [MIM:270450]. It is a disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain.
Post-translational
modifications
The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
Cellular localization
Membrane.
Target information above from: UniProt accessionP08069
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD221 antibody
- CD221 antibody
- CD221 antigen antibody
see all
Database links
- Entrez Gene: 3480 Human
- Entrez Gene: 16001 Mouse
- Entrez Gene: 25718 Rat
- Omim: 147370 Human
- SwissProt: P08069 Human
- SwissProt: Q60751 Mouse
- SwissProt: P24062 Rat
- Unigene: 643120 Human
see all
Anti-IGF1 Receptor (phospho Y1161) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - IGF1 Receptor (phospho Y1161) antibody (ab39398)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39398 diluted 1:50.
Immunohistochemistry (Paraffin-embedded sections) - IGF1 Receptor (phospho Y1161) antibody (ab39398)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39398 diluted 1:50 after pre-incubation with the immunogen (synthetic phosphopeptide)
Western blot - IGF1 Receptor (phospho Y1161) antibody (ab39398)
Predicted band size : 155 kDa
Western blot analysis using IGF1 Receptor antibody (ab39675, Lane 1, 2 and 3) and IGF1 Receptor (phospho-Tyr1161) antibody (ab39398, Lane 4 and 5).
Immunocytochemistry/ Immunofluorescence - IGF1 Receptor (phospho Y1161) antibody (ab39398)
ab39398 staining IGF1 Receptor in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody, at a 1/100 dilution, for 1 hour at 23°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
This image is courtesy of an anonymous abreview.
References for Anti-IGF1 Receptor (phospho Y1161) antibody (ab39398)
This product has been referenced in:
- Litzenburger BCet al. High IGF-IR activity in triple-negative breast cancer cell lines and tumorgrafts correlates with sensitivity to anti-IGF-IR therapy. Clin Cancer Res 17:2314-27 (2011). IHC-P; Human.Read more (PubMed: 21177763) »
- Honda Tet al. Upregulation of insulin-like growth factor binding protein 3 in astrocytes of transgenic mice that express Borna disease virus phosphoprotein. J Virol 85:4567-71 (2011). WB.Read more (PubMed: 21325425) »
See all 4 publications for this product
Publishing research using ab39398? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"