Overview

  • Product nameAnti-IGF2 antibody
    See all IGF2 primary antibodies
  • Description
    Rabbit polyclonal to IGF2
  • SpecificityMouse 20kDa IGF2 protein.Cross reacts with rat protein. Low cross reactivity was observed with human IGF2 protein.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse IGF2.
    Database link: P09535

Properties

Applications

Our Abpromise guarantee covers the use of ab63984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/200.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionThe insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • Involvement in diseaseEpigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • Sequence similaritiesBelongs to the insulin family.
  • Post-translational
    modifications
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf43 antibody
    • IGF 2 antibody
    • IGF II antibody
    • IGF-II antibody
    • IGF2 antibody
    • IGF2_HUMAN antibody
    • IGFII antibody
    • INSIGF antibody
    • insulin like growth factor 2 (somatomedin A) antibody
    • Insulin like Growth Factor 2 antibody
    • Insulin like growth factor II antibody
    • Insulin like growth factor II precursor antibody
    • Insulin like growth factor type 2 antibody
    • pp9974 antibody
    • Preptin antibody
    • putative insulin like growth factor II associated protein antibody
    • Somatomedin A antibody
    • Somatomedin-A antibody
    see all

Anti-IGF2 antibody images

  • Immunohistochemical analysis of murine amygdala tissue, staining IGF2 with ab63984 at 1/200 dilution. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody.

References for Anti-IGF2 antibody (ab63984)

This product has been referenced in:
  • Jung S  et al. Decreased expression of extracellular matrix proteins and trophic factors in the amygdala complex of depressed mice after chronic immobilization stress. BMC Neurosci 13:58 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22672618) »
  • Singh P  et al. Insulin-like growth factors are more effective than progastrin in reversing proapoptotic effects of curcumin: critical role of p38MAPK. Am J Physiol Gastrointest Liver Physiol 298:G551-62 (2010). Neutralising ; Rat . Read more (PubMed: 20133951) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate bufer
Sample Mouse Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Formaldehyde
Username

Dr. Suypriya Sen

Verified customer

Submitted May 13 2014

Abreviews
Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (Liver tissue)
Specification Liver tissue
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

Submitted May 13 2014

Thank you for contacting us.

We unfortunately do not offer trial size packages you have to buy the full volume of antibody.

Please note that in case this antibody does not work in applications (WB, IHC-P and IHC-Fr) in species (mouse)...

Read More

Thank you for contacting us.

I have checked the sequence homology (http://fasta.bioch.virginia.edu/fasta_www2/fasta_www.cgi) between Insulin (http://www.uniprot.org/uniprot/P01308) and IGF2 (http://www.uniprot.org/uniprot/P01344); though there...

Read More

Thank you for contacting us and for your interest in our products.

I am sorry to confirm that as far as we are aware, ab63984 has never been tested with samples from rat. All tested species cross-reactivity information covered by the guarant...

Read More

Thank you for contacting us. Please find the requested Certificate of Compliance attached. Please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"