Anti-IGF2 antibody (ab9574)
Key features and details
- Rabbit polyclonal to IGF2
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IGF2 antibody
See all IGF2 primary antibodies -
Description
Rabbit polyclonal to IGF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human IGF2. Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).
Database link: P01344 -
General notes
Although some customers have been successful in IHC we no longer batch test in this application.
Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (6) |
Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Sandwich ELISA |
Use at an assay dependent concentration.
To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. |
Notes |
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WB
Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich ELISA
Use at an assay dependent concentration. To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. |
Target
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Function
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
Involvement in disease
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
Sequence similarities
Belongs to the insulin family. -
Post-translational
modificationsO-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
see all
Images
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ab9574 detecting IGF2 using sandwich ELISA
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Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa
Lane 1: Marker
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (105)
ab9574 has been referenced in 105 publications.
- Sepúlveda D et al. Insulin-like growth factor 2 and autophagy gene expression alteration arise as potential biomarkers in Parkinson's disease. Sci Rep 12:2038 (2022). PubMed: 35132125
- Yu Y et al. Circ_0026579 alleviates LPS-induced WI-38 cells inflammation injury in infantile pneumonia. Innate Immun 28:37-48 (2022). PubMed: 35142578
- Yuan Z et al. Magnesium isoglycyrrhizinate suppresses bladder cancer progression by modulating the miR-26b/Nox4 axis. Bioengineered 13:7986-7999 (2022). PubMed: 35293283
- Lehman NL et al. Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes. Nat Commun 13:2083 (2022). PubMed: 35440587
- Catalano R et al. The cytoskeleton actin binding protein filamin A impairs both IGF2 mitogenic effects and the efficacy of IGF1R inhibitors in adrenocortical cancer cells. Cancer Lett 497:77-88 (2021). PubMed: 33075426