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Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).
Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types
Our Abpromise guarantee covers the use of ab9574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use at an assay dependent concentration. To detect hIGF-2 by ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-2.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-2 (10.0 ng/ml), a concentration of 3.1 - 4.6 µg/ml of this antibody is required.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
Immunohistochemical analysis of parafin embedded rat kidney tissue labelling IGF2 with ab9574 at 1ug/ml.
Image courtesy of an anonymous Abreview.
Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa
Lane 1: Marker
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng