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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Use a concentration of 5 µg/ml.
FunctionThe insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Involvement in diseaseEpigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
Sequence similaritiesBelongs to the insulin family.
Post-translational modificationsO-glycosylated with a core 1 or possibly core 8 glycan.
insulin like growth factor 2 (somatomedin A) antibody
Insulin like Growth Factor 2 antibody
Insulin like growth factor II antibody
Insulin like growth factor II precursor antibody
Insulin like growth factor type 2 antibody
putative insulin like growth factor II associated protein antibody
Somatomedin A antibody
Anti-IGF2 antibody images
Western blot - Anti-IGF2 antibody (ab99227)
All lanes : Anti-IGF2 antibody (ab99227) at 1 µg/ml
Lane 1 : IGF2 Human Recombinant Protein at 0.1 µg Lane 2 : IGF2 Human Recombinant Protein at 0.01 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa Observed band size : 47 kDa
Exposure time : 20 minutes
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab99227 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ICC/IF image of ab99227 stained Panc-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99227 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-IGF2 antibody (ab99227)
has not yet been referenced specifically in any publications.
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