Recombinant
RabMAb

Anti-IGF2 antibody [EPR12221] (ab177467)

Overview

  • Product name
    Anti-IGF2 antibody [EPR12221]
    See all IGF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12221] to IGF2
  • Specificity
    ab177467 has been found not to cross react with mouse or rat IGF-2 in western blot testing.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IGF2 aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P01344

  • Positive control
    • Human serum lysate and HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab177467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
      Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
    • Involvement in disease
      Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS) [MIM:180860]. A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age.
    • Sequence similarities
      Belongs to the insulin family.
    • Post-translational
      modifications
      O-glycosylated with core 1 or possibly core 8 glycans. Thr-96 is a minor glycosylation site compared to Thr-99.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C11orf43 antibody
      • FLJ22066 antibody
      • FLJ44734 antibody
      • GRDF antibody
      • IGF 2 antibody
      • IGF II antibody
      • IGF-II antibody
      • IGF2 antibody
      • IGF2_HUMAN antibody
      • IGFII antibody
      • INSIGF antibody
      • Insulin like growth factor 2 (somatomedin A) antibody
      • Insulin like Growth Factor 2 antibody
      • Insulin like growth factor II antibody
      • Insulin like growth factor II precursor antibody
      • Insulin like growth factor type 2 antibody
      • pp9974 antibody
      • Preptin antibody
      • Putative insulin like growth factor II associated protein antibody
      • Somatomedin A antibody
      • Somatomedin-A antibody
      • T3M 11 derived growth factor antibody
      see all

    Images

    • Immunofluorescence analysis of HepG2 cells labeling IGF2  (green)  with ab177467 at 1/50 dilution. DAPI nuclear staining (blue).

    • Anti-IGF2 antibody [EPR12221] (ab177467) at 1/1000 dilution + Human serum lysate at 10 µg

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 20 kDa

    References

    ab177467 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    We haven't tested the cross-reactivity of ab177467 with IGF-1.
    However, based on the the immunogen sequence analysis the homology of the immunogen peptide is only 50% and no continuous sequences more than 3 aa could be found.
    It seems tha...

    Read More

    The laboratory completed the blocking study for ab177467 (IGF2), and found that the blocking peptide did successfully block the 50 kDa band, indicating that this band is associated with the immunogen.

    We found the 2 attached papers, which cite...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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