Overview

  • Product name
    Anti-IGF2 antibody [mAbcam63294]
    See all IGF2 primary antibodies
  • Description
    Mouse monoclonal [mAbcam63294] to IGF2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from within residues 150 to the C-terminus of Human IGF2.

  • Positive control
    • This antibody gave a positive signal with a full-length, recombinant IGF2 protein.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab63294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • Involvement in disease
    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • Sequence similarities
    Belongs to the insulin family.
  • Post-translational
    modifications
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf43 antibody
    • IGF 2 antibody
    • IGF II antibody
    • IGF-II antibody
    • IGF2 antibody
    • IGF2_HUMAN antibody
    • IGFII antibody
    • INSIGF antibody
    • insulin like growth factor 2 (somatomedin A) antibody
    • Insulin like Growth Factor 2 antibody
    • Insulin like growth factor II antibody
    • Insulin like growth factor II precursor antibody
    • Insulin like growth factor type 2 antibody
    • pp9974 antibody
    • Preptin antibody
    • putative insulin like growth factor II associated protein antibody
    • Somatomedin A antibody
    • Somatomedin-A antibody
    see all

Images

  • Anti-IGF2 antibody [mAbcam63294] (ab63294) at 1 µg/ml + IGF2 Human Recombinant Protein at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    Human full length recombinant protein with GST tag (MW-46 kDa with tag).

References

This product has been referenced in:
  • Leslie K  et al. Increased apoptosis, altered oxygen signaling, and antioxidant defenses in first-trimester pregnancies with high-resistance uterine artery blood flow. Am J Pathol 185:2731-41 (2015). WB ; Human . Read more (PubMed: 26362067) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab63294.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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