Overview

  • Product nameAnti-IGF2BP1 antibody
    See all IGF2BP1 primary antibodies
  • Description
    Rabbit polyclonal to IGF2BP1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (KSGYAFVDCP DEHWAMKAIE TFSGKVELQG KRLEIEHSVP KKQRSRKIQI) of human IGF2BP1 (NP_006537).

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab82968 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/300.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.Can be blocked with Human IGF2BP1 peptide (ab100852). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRNA-binding factor that affects mRNA nuclear export, localization, stability and translation. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Regulates mRNA stability during the integrated cellular stress response (ISR) in stress granules (SGs). Stabilizes the BTRC/FBW1A mRNA from degradation by disrupting miRNA-dependent interaction with AGO2. Identified in a HCV IRES-mediated translation complex, that enhances translation at the Hepatitis C virus (HCV) RNA-replicon via the internal ribosome entry site (IRES), but does not affect 5'cap-dependent translation. Acts as a HIV-1 retrovirus restriction factor that reduces HIV-1 assembly by inhibiting viral RNA packaging, assembly and processing of HIV-1 GAG protein on cellular membranes. Binds to mRNAs in stress granules (SGs). Binds to the stem-loop IV of the 5'-UTR and to the variable region and the poly(U-C) motif of the 3'-UTR of the HCV RNA-replicon. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulates its subcellular localization and translation. Binds both to the coding region mRNA stability determinant (CRD) and to AU-rich sequences in the 3'-UTR of the MYC and CD44 mRNAs and stabilizes these mRNAs. Binds to the fourth and fifth exons of the oncofetal H19 and neuron-specific TAU mRNAs and regulates their localizations. Binds to the adenine-rich autoregulatory sequence (ARS) 5'-UTR of the PABPC1 mRNA and is involved in its translational repression. The RNA-binding activity to ARS is stimulated by PABPC1. Binds to the coding sequence region of BTRC/FBW1A mRNA and mediates stabilization of BTRC/FBW1A and MYC mRNAs in response to beta-catenin signaling. Binding to RNA employs a cooperative, sequential mechanism of homo- or heterodimerisation. Also involved in growth or survival of lung-cancer cells. Protects the MYC and MDR-1 mRNAs from cleavage by a endoribonuclease, thus prolonging their stabilities (By similarity). Binds to the 3'-UTR axonal localization signal (ALS) of TAU mRNA (By similarity). Binds to a conserved 54-nucleotide element in the 3'-UTR of the beta actin mRNA known as the 'zipcode' (By similarity). Promotes translocation of the beta-actin mRNA to dendrites (By similarity). May act as a regulator of mRNA transport to activated synapses in response to synaptic activity.
  • Tissue specificityExpressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary, gonocytes of testis, oocytes, spermatogonia and semen (at protein level). Expressed in testicular and lung cancer (at protein level). Expressed in kidney, prostate, trachea, testis and lung cancer.
  • Sequence similaritiesBelongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • DomainThe third and fourth KH domains encompass the protein dimerization motif and are necessary and sufficient for RNA binding. The four KH domains are important for granule formation and SGs targeting. Contains two nuclear export signals, situated within the second and fourth KH domains. The four KH domains are important to suppress HIV-1 infectivity.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation may influence mRNA translation.
  • Cellular localizationNucleus. Cytoplasm. Cell projection > lamellipodium. Cell projection > dendrite. Cell projection > dendritic spine. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Colocalized with H19 RNA at lamellipodia. Colocalized with CD44 mRNA in RNP granules. Nuclear export is mediated by XPO1/CRM1. In motile cells, is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). Present in the form of granules and into F-actin-rich protrusion of dendrites, spines and subsynaptic sites (By similarity). Colocalizes with beta-actin mRNA in dendrites and spines (By similarity). Exhibited rapid, bidirectional movements in dendrites and spines (By similarity). Neuronal depolarization by KCl induces its rapid efflux from the cell body into dendrites.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coding region determinant-binding protein antibody
    • CRD BP antibody
    • CRD-BP antibody
    • CRDBP antibody
    • IF2B1 antibody
    • IF2B1_HUMAN antibody
    • IGF II mRNA binding protein 1 antibody
    • IGF-II mRNA-binding protein 1 antibody
    • IGF2 mRNA binding protein 1 antibody
    • IGF2 mRNA-binding protein 1 antibody
    • IGF2BP1 antibody
    • IMP 1 antibody
    • IMP-1 antibody
    • IMP1 antibody
    • Insulin like growth factor 2 mRNA binding protein 1 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 1 antibody
    • VICKZ family member 1 antibody
    • VICKZ1 antibody
    • ZBP 1 antibody
    • ZBP-1 antibody
    • ZBP1 antibody
    • Zip code binding protein 1 antibody
    • Zip code-binding protein 1 antibody
    • Zipcode binding protein 1 antibody
    • Zipcode-binding protein 1 antibody
    see all

Anti-IGF2BP1 antibody images

  • Sample Type : Human lung carcinoma. Primary Antibody Dilution : 1:300. Secondary Antibody : Anti-rabbit-linker. Fbex-HRP. Color/Signal Descriptions : Brown: IGFBP1 Blue: Nuclei. Gene Name : IGF2BP1.
  • Application: IHC. Species+tissue/cell type: Human small intestine. Primary antibody dilution: 1:300. Secondary antibody: Anti-rabbit-linker. Fbex-HRP
  • Anti-IGF2BP1 antibody (ab82968) at 1 µg/ml (in 5% skim milk / PBS buffer) + jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Lanes 1 & 4 : Anti-IGF2BP1 antibody (ab82968) at 1/1000 dilution (in 5% milk for 1 1/2 hours at 25°C )
    Lanes 2 & 5 : Anti-IGF2BP1 antibody (ab82968) at 1/1000 dilution (in 0.5% milk for 1 1/2 hours at 25°C )
    Lanes 3 & 6 : Anti-IGF2BP1 antibody (ab82968) at 1/10000 dilution (in 0.5% milk for 1 1/2 hours at 25°C )

    Lane 1 : Whole cell lysate of Dog Osteosarcoma (high-expressing cell lines) blocked with 5% milk in TBST for 15 hours at 4°C
    Lane 2 : Whole cell lysate of Dog Osteosarcoma (high-expressing cell lines) blocked with 0.5% milk in TBST for 15 hours at 4°C
    Lane 3 : Whole cell lysate of Dog Osteosarcoma (high-expressing cell lines) blocked with 0.5% milk in TBST for 15 hours at 4°C
    Lane 4 : Whole cell lysate of Dog Osteosarcoma (low-expressing cell lines) blocked with 5% milk in TBST for 15 hours at 4°C
    Lane 5 : Whole cell lysate of Dog Osteosarcoma (low-expressing cell lines) blocked with 0.5% milk in TBST for 15 hours at 4°C
    Lane 6 : Whole cell lysate of Dog Osteosarcoma (low-expressing cell lines) blocked with 0.5% milk in TBST for 15 hours at 4°C

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 28 minutes

    This image is courtesy of an Abreview submitted by Liza O'Donoghue

    No bands were visible at the higher dilution, even with a stronger detection substrate.

    See Abreview

  • ab82968 staining IGF2BP1 in dog kidney, sebaceous gland, and epidermis by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with formalin, an antigen retrieval step was performed using a pH6 antigen retrieval solution. Tissue was then blocked followed by incubation with the primary antibody, at a 1/500 dilution, for 16 hours at 4°C. A Biotin-conjugated goat polyclonal was used as secondary antibody at a 1/1000 dilution.
    Note that the brown staining in the epidermis section minus primary antibody is natural pigment, not inapropriate antibody binding.

    See Abreview

References for Anti-IGF2BP1 antibody (ab82968)

This product has been referenced in:
  • Fakhraldeen SA  et al. Two Isoforms of the RNA Binding Protein, Coding Region Determinant-binding Protein (CRD-BP/IGF2BP1), Are Expressed in Breast Epithelium and Support Clonogenic Growth of Breast Tumor Cells. J Biol Chem 290:13386-400 (2015). WB, ICC/IF ; Mouse . Read more (PubMed: 25861986) »
  • Li W  et al. Role of IGF2BP3 in trophoblast cell invasion and migration. Cell Death Dis 5:e1025 (2014). WB ; Human . Read more (PubMed: 24457969) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (MEL, 293T cell)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 20 µg
Specification MEL, 293T cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 08 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - tween-20
Specification HeLa
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted May 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Columnar epithelial cells of normal colon mucosa)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Permeabilization Yes - tween-20
Specification Columnar epithelial cells of normal colon mucosa
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 11 2015

Application Western blot
Sample Human Cell lysate - whole cell (hela, a431, k562)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 20 µg
Specification hela, a431, k562
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 30 2015

Thank you for contacting us and your interest in our products.

The anti-IGF2BP1 antibody (ab82968) your customer is hoping to use has been applied in a dilution of 1/500 by a previous customer. The result of their experiment in using the anti...

Read More

Thank you for your inquiry.

I can confirm that ab82968 and ab92355 both can be used with citrate buffer pH6 as antigen retrieval. That is according to a n Abreview and lab testing respectively.

ab83215 is not tested and guaranteed f...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Kidney, sebaceous gland, epidermis)
Specification Kidney, sebaceous gland, epidermis
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Vector pH6 Ag Retrieval solution
Permeabilization No
Blocking step Biocare Background Sniper and Vector Avidin/Biotin blocking kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Ms. Liza O'Donoghue

Verified customer

Submitted Sep 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Osteosarcoma cell lines)
Loading amount 50 µg
Specification Osteosarcoma cell lines
Gel Running Conditions Reduced Denaturing (10% with 5% stacking gel)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 4°C
Username

Ms. Liza O'Donoghue

Verified customer

Submitted Jul 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"