Overview

  • Product name
    Anti-IGF2BP2 antibody
    See all IGF2BP2 primary antibodies
  • Description
    Rabbit polyclonal to IGF2BP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-260 of Human IGF2BP2 (Uniprot Q9Y6M1).

  • Positive control
    • 293T, A431 and HepG2 whole cell lysates; HeLa cells, Human colon cancer tissue.
  • General notes
    Store as concentrated solution

Properties

Applications

Our Abpromise guarantee covers the use of ab151463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval buffer: Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
  • Tissue specificity
    Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
  • Sequence similarities
    Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain
    The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
  • Cellular localization
    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
  • Information by UniProt
  • Database links
    • Alternative names
      • Hepatocellular carcinoma autoantigen p62 antibody
      • IF2B2_HUMAN antibody
      • IGF 2 mRNA binding protein 2 antibody
      • IGF II mRNA binding protein 2 antibody
      • IGF-II mRNA-binding protein 2 antibody
      • IGF2 mRNA-binding protein 2 antibody
      • Igf2bp2 antibody
      • IMP 2 antibody
      • IMP-2 antibody
      • IMP2 antibody
      • Insulin like growth factor 2 mRNA binding protein 2 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
      • OTTHUMP00000082606 antibody
      • OTTHUMP00000082607 antibody
      • OTTHUMP00000082608 antibody
      • p62 antibody
      • VICKZ 2 antibody
      • VICKZ family member 2 antibody
      • VICKZ2 antibody
      see all

    Images

    • Western blot - Anti-IGF2BP2 antibody (ab151463)
      Western blot - Anti-IGF2BP2 antibody (ab151463)
      All lanes : Anti-IGF2BP2 antibody (ab151463) at 1/3000 dilution

      Lane 1 : 293T whole cell lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 66 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP2 antibody (ab151463)
      Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP2 antibody (ab151463)
      Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labelling IGF2BP2 with ab151463 at 1/500 dilution. Right hand image shows ab151463 staining (green) overlaid with Hoechst 33343 (blue).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2 antibody (ab151463)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2 antibody (ab151463)
      Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labelling IGF2BP2 with 151463 at 1/250 dilution.

    References

    ab151463 has not yet been referenced specifically in any publications.

    Publishing research using ab151463? Please let us know so that we can cite the reference in this datasheet.

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