Overview

  • Product nameAnti-IGF2BP2 antibody
    See all IGF2BP2 primary antibodies
  • Description
    Mouse polyclonal to IGF2BP2
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length IGF2BP2 protein (Human)

  • Positive control
    • IGF2BP2 transfected 293T cell lysate and human placenta tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects bands of approximately 55 and 75 kDa (predicted molecular weight: 62 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
    • Tissue specificityExpressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
    • Sequence similaritiesBelongs to the RRM IMP/VICKZ family.
      Contains 4 KH domains.
      Contains 2 RRM (RNA recognition motif) domains.
    • DomainThe third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
    • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hepatocellular carcinoma autoantigen p62 antibody
      • IF2B2_HUMAN antibody
      • IGF 2 mRNA binding protein 2 antibody
      • IGF II mRNA binding protein 2 antibody
      • IGF-II mRNA-binding protein 2 antibody
      • IGF2 mRNA-binding protein 2 antibody
      • Igf2bp2 antibody
      • IMP 2 antibody
      • IMP-2 antibody
      • IMP2 antibody
      • Insulin like growth factor 2 mRNA binding protein 2 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
      • OTTHUMP00000082606 antibody
      • OTTHUMP00000082607 antibody
      • OTTHUMP00000082608 antibody
      • p62 antibody
      • VICKZ 2 antibody
      • VICKZ family member 2 antibody
      • VICKZ2 antibody
      see all

    Anti-IGF2BP2 antibody images

    • Anti-IGF2BP2 antibody (ab67434) at 1/500 dilution + human placenta tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 62 kDa
      Observed band size : 62 kDa
    • All lanes : Anti-IGF2BP2 antibody (ab67434) at 1/500 dilution

      Lane 1 : IGF2BP2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 62 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab67434 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67434, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-IGF2BP2 antibody (ab67434)

    ab67434 has not yet been referenced specifically in any publications.

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