Anti-IGF2BP2 antibody [EPR6741(B)] (ab124930)

Overview

  • Product nameAnti-IGF2BP2 antibody [EPR6741(B)]
    See all IGF2BP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6741(B)] to IGF2BP2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human IGF2BP2.

  • Positive control
    • 293T, Caco2, HepG2, and SH SY5Y cell lysates; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-IGF2BP2 antibody (Alexa Fluor® 647) [EPR6741(B)] (ab206607)

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55-65 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionBinds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
    • Tissue specificityExpressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
    • Sequence similaritiesBelongs to the RRM IMP/VICKZ family.
      Contains 4 KH domains.
      Contains 2 RRM (RNA recognition motif) domains.
    • DomainThe third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
    • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hepatocellular carcinoma autoantigen p62 antibody
      • IF2B2_HUMAN antibody
      • IGF 2 mRNA binding protein 2 antibody
      • IGF II mRNA binding protein 2 antibody
      • IGF-II mRNA-binding protein 2 antibody
      • IGF2 mRNA-binding protein 2 antibody
      • Igf2bp2 antibody
      • IMP 2 antibody
      • IMP-2 antibody
      • IMP2 antibody
      • Insulin like growth factor 2 mRNA binding protein 2 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
      • OTTHUMP00000082606 antibody
      • OTTHUMP00000082607 antibody
      • OTTHUMP00000082608 antibody
      • p62 antibody
      • VICKZ 2 antibody
      • VICKZ family member 2 antibody
      • VICKZ2 antibody
      see all

    Anti-IGF2BP2 antibody [EPR6741(B)] images

    • All lanes : Anti-IGF2BP2 antibody [EPR6741(B)] (ab124930) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Caco2 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 66 kDa
    • ab124930, at 1/50 dilution, staining GF2BP2 in formalin-fixed, paraffin-embedded Human colon tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-IGF2BP2 antibody [EPR6741(B)] (ab124930)

    ab124930 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"