The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: 1/500. Precipitates >90% of 10ng IGFBP 1.
WB: 1/1000. Detects 10ng SDS denatured human IGFBP 1. Predicted molecular weight: 30 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.