The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: 4µl immunoprecipitated IGFBP 2 from 600µl of concentrated T98G serum free culture supernatant.
WB: 1/1000 - 1/2000. This detects IGFBP 2 in concentrated T98G serum free culture supernatant. T98G cell culture supernatant was resolved by electrophoresis, transferred to nitrocellulose and probed with anti IGFBP 2 (1/1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 32kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.