Overview

  • Product nameAnti-IGFBP2 antibody
    See all IGFBP2 primary antibodies
  • Description
    Rabbit polyclonal to IGFBP2
  • SpecificityRecognizes bovine IGFBP 2 at 32kD. Slight cross reactivity with IGFBP 1, 3, 4 and 5.
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Goat, Horse, Hamster, Cow, Human
  • Immunogen

    Bovine IGFBP 2 purified by immunoaffinity and HPLC chromatographies from conditioned medium of Madin Darby Bovine Kidney cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab4244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notesIP: 4µl immunoprecipitated IGFBP 2 from 600µl of concentrated T98G serum free culture supernatant.
    WB: 1/1000 - 1/2000. This detects IGFBP 2 in concentrated T98G serum free culture supernatant. T98G cell culture supernatant was resolved by electrophoresis, transferred to nitrocellulose and probed with anti IGFBP 2 (1/1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 32kDa.
    Not tested in other applications.

    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Sequence similaritiesContains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • DomainThe C-terminus is required for IGF-binding and growth inhibition.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • BP 2 antibody
      • BP2 antibody
      • IBP 2 antibody
      • IBP-2 antibody
      • IBP2 antibody
      • IBP2_HUMAN antibody
      • IGF binding protein 2 antibody
      • IGF BP53 antibody
      • IGF-binding protein 2 antibody
      • IGFBP 2 antibody
      • IGFBP-2 antibody
      • IGFBP2 antibody
      • IGFBP53 antibody
      • Insulin like growth factor binding protein 2 36kDa antibody
      • Insulin like growth factor binding protein 2 antibody
      • Insulin like growth factor-binding protein 2 precursor antibody
      • Insulin-like growth factor-binding protein 2 antibody
      see all

    References for Anti-IGFBP2 antibody (ab4244)

    This product has been referenced in:
    • Lawlor K  et al. Pathway-based biomarker search by high-throughput proteomics profiling of secretomes. J Proteome Res 8:1489-503 (2009). Read more (PubMed: 19199430) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (eye)
    Specification eye
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: buffer
    Permeabilization No
    Username

    Mrs. Jinsuk An

    Verified customer

    Submitted May 20 2009

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (glioma cell -line U87MG)
    Loading amount 50 µg
    Specification glioma cell -line U87MG
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Mr. Paul Eve

    Verified customer

    Submitted Jan 15 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"