Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab91404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Predicted molecular weight: 35 kDa.
ELISA 1/1000.

Target

  • FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • DomainThe C-terminus is required for IGF-binding and growth inhibition.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BP 2 antibody
    • BP2 antibody
    • IBP 2 antibody
    • IBP-2 antibody
    • IBP2 antibody
    • IBP2_HUMAN antibody
    • IGF binding protein 2 antibody
    • IGF BP53 antibody
    • IGF-binding protein 2 antibody
    • IGFBP 2 antibody
    • IGFBP-2 antibody
    • IGFBP2 antibody
    • IGFBP53 antibody
    • Insulin like growth factor binding protein 2 36kDa antibody
    • Insulin like growth factor binding protein 2 antibody
    • Insulin like growth factor-binding protein 2 precursor antibody
    • Insulin-like growth factor-binding protein 2 antibody
    see all

Anti-IGFBP2 antibody images

  • Anti-IGFBP2 antibody (ab91404) at 2 µg/ml + Jurkat cell lysate at 35 µg

    Predicted band size : 35 kDa
  • Anti-IGFBP2 antibody (ab91404) at 1/500 dilution + Recombinant human IGFBP2 protein (ab63223) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds
  • Formalin-fixed and paraffin-embedded human brain tissue with ab91404, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • Confocal immunofluorescent analysis of ab91404 with A549 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • Flow cytometric analysis of jurkat cells using ab91404 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References for Anti-IGFBP2 antibody (ab91404)

ab91404 has not yet been referenced specifically in any publications.

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Es tut mir leid, dass Sie Probleme mit diesem IGFBP2-Antikörper hatten.Ich weiß, dass Sie schon viel Zeit in Ihre Experimente investiert haben und es ist enttäuschend, dass Sie bisher noch ke...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"