Recombinant
RabMAb

Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Overview

  • Product name
    Anti-IGFBP2 antibody [EPR18012-257]
    See all IGFBP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR18012-257] to IGFBP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, IP, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse IGFBP2 aa 1-250. The exact sequence is proprietary.
    Database link: Q91VK7

  • Positive control
    • WB: Human, rat and mouse serum; human liver lysate; T-47D and RAW 264.7 whole cell lysate. IHC-P: Mouse choroid plexus and liver tissue. IHC-Fr: Mouse and rat brain (choroid plexus). Flow Cytometry: T-47D and RAW 264.7 cells. IP: Human serum.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab188200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
IP 1/30.
WB 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This antibody is not suitable for human and rat species in IHC application due to non-specific or negative staining.

Flow Cyt 1/60.

Target

  • Function
    Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similarities
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Domain
    The C-terminus is required for IGF-binding and growth inhibition.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BP 2 antibody
    • BP2 antibody
    • IBP 2 antibody
    • IBP-2 antibody
    • IBP2 antibody
    • IBP2_HUMAN antibody
    • IGF binding protein 2 antibody
    • IGF BP53 antibody
    • IGF-binding protein 2 antibody
    • IGFBP 2 antibody
    • IGFBP-2 antibody
    • IGFBP2 antibody
    • IGFBP53 antibody
    • Insulin like growth factor binding protein 2 36kDa antibody
    • Insulin like growth factor binding protein 2 antibody
    • Insulin like growth factor-binding protein 2 precursor antibody
    • Insulin-like growth factor-binding protein 2 antibody
    see all

Images

  • All lanes : Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution

    Lane 1 : Human serum
    Lane 2 : Human liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 3 minutes


    Blocking: 5% NFDM/TBST.

    The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.

  • Immunohistochemical analysis of paraffin-embedded mouse choroid plexus tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse choroid plexus (PMID: 7525264; PMID: 7678219) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized mouse brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on mouse tissue section is observed. Counter stained with DAPI.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
    Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).

  • Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • IGFBP2 was immunoprecipitated from 0.35 mg of human serum with ab188200 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188200 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: Human serum  10 μg (Input).
    Lane 2: ab188200 IP in Human serum (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of  ab188200 in human serum (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.

  • All lanes : Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
    Lane 3 : Rat serum
    Lane 4 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 3 minutes


    Blocking: 5% NFDM/TBST.

    The expression profile observed on T-47D is consistent with what has been described in the literature (PMID: 23515291).

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse liver (PMID: 7678219) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized rat brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on rat tissue section is observed. Counter stained with DAPI.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
    Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).

  • Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

References

ab188200 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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