Overview

  • Product nameAnti-IGFBP2 antibody [EPR3380(2)]
    See all IGFBP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3380(2)] to IGFBP2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues at the C-terminus of Human IGFBP2.

  • Positive control
    • Human kidney tissue, Human endometrial adenocarcinoma tissue. T47-D, SH-SY5Y, BxPC-3, A549 and NCCIT cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Sequence similaritiesContains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • DomainThe C-terminus is required for IGF-binding and growth inhibition.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • BP 2 antibody
      • BP2 antibody
      • IBP 2 antibody
      • IBP-2 antibody
      • IBP2 antibody
      • IBP2_HUMAN antibody
      • IGF binding protein 2 antibody
      • IGF BP53 antibody
      • IGF-binding protein 2 antibody
      • IGFBP 2 antibody
      • IGFBP-2 antibody
      • IGFBP2 antibody
      • IGFBP53 antibody
      • Insulin like growth factor binding protein 2 36kDa antibody
      • Insulin like growth factor binding protein 2 antibody
      • Insulin like growth factor-binding protein 2 precursor antibody
      • Insulin-like growth factor-binding protein 2 antibody
      see all

    Anti-IGFBP2 antibody [EPR3380(2)] images

    • All lanes : Anti-IGFBP2 antibody [EPR3380(2)] (ab109284) at 1/10000 dilution

      Lane 1 : T47-D cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : BxPC-3 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
    • Immunohistochemical analysis of paraffin-embedded kidney tissue using 1/100 ab109284.
    • Immunohistochemical analysis of paraffin-embedded endometrial adenocarcinoma tissue with ab109284 at 1/100 dilution.

    References for Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)

    This product has been referenced in:
    • Wang C  et al. Human adipocytes stimulate invasion of breast cancer MCF-7 cells by secreting IGFBP-2. PLoS One 10:e0119348 (2015). WB ; Human . Read more (PubMed: 25747684) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"