• Product nameAnti-IGFBP3 antibody
    See all IGFBP3 primary antibodies
  • Description
    Mouse monoclonal to IGFBP3
  • SpecificityAb36529 is specific to IGFBP3. It shows less than 0.05% reactivity with human IGFBPs 1, 2, 4, 5 or 6, as determined by EIA.
  • Tested applicationsSuitable for: ELISA, WB, Dot Blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    IGFBP3 plus epitope of human IGFBP3.



Our Abpromise guarantee covers the use of ab36529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot
  • Application notesDot: 1/5000.
    ELISA: 1/8000.
    WB: A 1/5000 dilution detects 60ng IGFBP3. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Tissue specificityExpressed by most tissues. Present in plasma.
    • Sequence similaritiesContains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Developmental stageIGFBP3 levels are higher during extrauterine life and peak during puberty.
    • DomainThe thyroglobulin type-1 domain mediates interaction with HN.
    • Post-translational
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Anti-IGFBP3 antibody images

    • ab36529 staining human fibroblast cells by ICC/IF.  Cells were fixed in formaldehyde, permeabilised in 0.5% triton for 5 min, and blocked with 4% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 with 4% BSA/TBST and incubated for 2 hours at 20°C.  An Alexa Fluor® 594 conjugated donkey anti-mouse was used as the secondary antibody.

      Red fluorescence shows IGFBP3, blue is DNA.

      See Abreview

    References for Anti-IGFBP3 antibody (ab36529)

    This product has been referenced in:
    • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . Read more (PubMed: 19639960) »
    • Heath KE  et al. Primary acid-labile subunit deficiency due to recessive IGFALS mutations results in postnatal growth deficit associated with low circulating insulin growth factor (IGF)-I, IGF binding protein-3 levels, and hyperinsulinemia. J Clin Endocrinol Metab 93:1616-24 (2008). Read more (PubMed: 18303074) »

    See all 2 Publications for this product

    Product Wall

    I received some information regarding the immunogen of this product and thought that you might be interested in knowing. The synthetic peptide used as an immunogen for this antibody was derived from the central domain of IGFBP-3 and does not contai...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (fibroblast)
    Specification fibroblast
    Fixative Formaldehyde
    Permeabilization Yes - 0.5% triton for 5 min
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C

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    Submitted Jan 09 2008