Overview

  • Product name
  • Description
    Mouse monoclonal to IGFBP3
  • Specificity
    Ab36529 is specific to IGFBP3. It shows less than 0.05% reactivity with human IGFBPs 1, 2, 4, 5 or 6, as determined by EIA.
  • Tested applications
    Suitable for: ELISA, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    IGFBP3 plus epitope of human IGFBP3.

Properties

Applications

Our Abpromise guarantee covers the use of ab36529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Dot blot
  • Application notes
    Dot: 1/5000.
    ELISA: 1/8000.
    WB: A 1/5000 dilution detects 60ng IGFBP3. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Tissue specificity
      Expressed by most tissues. Present in plasma.
    • Sequence similarities
      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Developmental stage
      IGFBP3 levels are higher during extrauterine life and peak during puberty.
    • Domain
      The thyroglobulin type-1 domain mediates interaction with HN.
    • Post-translational
      modifications
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Images

    • ab36529 staining human fibroblast cells by ICC/IF.  Cells were fixed in formaldehyde, permeabilised in 0.5% triton for 5 min, and blocked with 4% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 with 4% BSA/TBST and incubated for 2 hours at 20°C.  An Alexa Fluor® 594 conjugated donkey anti-mouse was used as the secondary antibody.

      Red fluorescence shows IGFBP3, blue is DNA.

      See Abreview

    References

    This product has been referenced in:
    • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . Read more (PubMed: 19639960) »
    • Heath KE  et al. Primary acid-labile subunit deficiency due to recessive IGFALS mutations results in postnatal growth deficit associated with low circulating insulin growth factor (IGF)-I, IGF binding protein-3 levels, and hyperinsulinemia. J Clin Endocrinol Metab 93:1616-24 (2008). Read more (PubMed: 18303074) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    I received some information regarding the immunogen of this product and thought that you might be interested in knowing. The synthetic peptide used as an immunogen for this antibody was derived from the central domain of IGFBP-3 and does not contai...

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblast)
    Specification
    fibroblast
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% triton for 5 min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 09 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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