Overview

  • Product name
  • Description
    Rabbit polyclonal to IGFBP3
  • Specificity
    Recognizes human IGFBP 3. Slight crossreactivity with other IGF binding proteins: IGFBP 1: ~10%, IGFBP 2: <0.2%, IGFBP 4: <0.2%, IGFBP 5: <0.2%.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat, Horse, Cow, Pig
  • Immunogen

    Recombinant full length protein (Human IGFBP 3 expressed in CHO cells).

Applications

Our Abpromise guarantee covers the use of ab4248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/1000. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.

Target

  • Function
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Tissue specificity
    Expressed by most tissues. Present in plasma.
  • Sequence similarities
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Developmental stage
    IGFBP3 levels are higher during extrauterine life and peak during puberty.
  • Domain
    The thyroglobulin type-1 domain mediates interaction with HN.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acid stable subunit of the 140 K IGF complex antibody
    • Binding protein 29 antibody
    • Binding protein 53 antibody
    • BP 53 antibody
    • BP53 antibody
    • Growth hormone dependent binding protein antibody
    • IBP 3 antibody
    • IBP-3 antibody
    • IBP3 antibody
    • IBP3_HUMAN antibody
    • IGF binding protein 3 antibody
    • IGF-binding protein 3 antibody
    • IGFBP 3 antibody
    • IGFBP-3 antibody
    • IGFBP3 antibody
    • Insulin Like Growth Factor Binding Protein 3 antibody
    • Insulin-like growth factor binding protein 3 precursor antibody
    • Insulin-like growth factor-binding protein 3 antibody
    see all

References

This product has been referenced in:
  • Walker G  et al. Insulin-like growth factor binding proteins IGFBP3, IGFBP4, and IGFBP5 predict endocrine responsiveness in patients with ovarian cancer. Clin Cancer Res 13:1438-44 (2007). Read more (PubMed: 17332286) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. We are happy to provide quote for 400 micro-litre of this antibody. The concentration of this antibody was not determined so we will be unable to give you quote for 400 µg. We generally do not determine the concentratio...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Specification
fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% triton for 5 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 18 2008

Thank you for your patience in awaiting a response. Having discussed this issue with my colleagues and the originator of the product. The following may explain the route cause of the problem I have been told that in all cases, the IGFBP's are "stick...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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