• Product nameAnti-IGFBP3 antibody
    See all IGFBP3 primary antibodies
  • Description
    Goat polyclonal to IGFBP3
  • Specificityab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Sheep, Human, Pig
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog
  • Immunogen

    Synthetic peptide:


    , from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2).

  • Positive control
    • Human breast cancer lysate.



Our Abpromise guarantee covers the use of ab77635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Tissue specificityExpressed by most tissues. Present in plasma.
    • Sequence similaritiesContains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Developmental stageIGFBP3 levels are higher during extrauterine life and peak during puberty.
    • DomainThe thyroglobulin type-1 domain mediates interaction with HN.
    • Post-translational
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Anti-IGFBP3 antibody images

    • All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml

      Lane 1 : human breast cancer lysate (in RIPA buffer)
      Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide

      Lysates/proteins at 35 µg per lane.

      Predicted band size : 32 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible non-specific binding).

    References for Anti-IGFBP3 antibody (ab77635)

    This product has been referenced in:
    • Marthandan S  et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016). Read more (PubMed: 27464526) »
    • Goyal R  et al. Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries. PLoS One 8:e82200 (2013). WB ; Sheep . Read more (PubMed: 24367503) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Sheep Tissue lysate - whole (Liver)
    Loading amount 3 µg
    Specification Liver
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Sep 21 2011