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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab77635 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Tissue specificity Expressed by most tissues. Present in plasma.
Sequence similarities Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.
Developmental stage IGFBP3 levels are higher during extrauterine life and peak during puberty.
Domain The thyroglobulin type-1 domain mediates interaction with HN.
Post-translational modifications Phosphorylation sites are present in the extracelllular medium.
Cellular localization Secreted.
Information by UniProt
Acid stable subunit of the 140 K IGF complex antibody
Binding protein 29 antibody
Binding protein 53 antibody
Anti-IGFBP3 antibody images
Western blot - IGFBP3 antibody (ab77635)
All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml Lane 1 : human breast cancer lysate (in RIPA buffer) Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide Lysates/proteins at 35 µg per lane. Predicted band size : 32 kDa Observed band size : 28 kDa ( why is the actual band size different from the predicted?) Additional bands at : 50 kDa (possible non-specific binding).
References for Anti-IGFBP3 antibody (ab77635)
This product has been referenced in:
et al. Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries. PLoS One 8:e82200 (2013).
Read more (PubMed: 24367503) »
et al. Insulin-like growth factor-binding protein-3 mediates high glucose-induced apoptosis by increasing oxidative stress in proximal tubular epithelial cells. Endocrinology 152:3135-42 (2011).
Read more (PubMed: 21652730) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"