Overview

  • Product name
  • Description
    Goat polyclonal to IGFBP3
  • Specificity
    ab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Sheep, Human, Pig
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-RYKVDYESQSTDTQN

    , from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2).

  • Positive control
    • Human breast cancer lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Tissue specificity
      Expressed by most tissues. Present in plasma.
    • Sequence similarities
      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Developmental stage
      IGFBP3 levels are higher during extrauterine life and peak during puberty.
    • Domain
      The thyroglobulin type-1 domain mediates interaction with HN.
    • Post-translational
      modifications
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Images

    • All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml

      Lane 1 : human breast cancer lysate (in RIPA buffer)
      Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 32 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible non-specific binding).

    References

    This product has been referenced in:
    • Marthandan S  et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016). Read more (PubMed: 27464526) »
    • Goyal R  et al. Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries. PLoS One 8:e82200 (2013). WB ; Sheep . Read more (PubMed: 24367503) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Liver)
    Loading amount
    3 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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