Anti-IGFBP3 antibody (Biotin) (ab84228)

Overview

  • Product nameAnti-IGFBP3 antibody (Biotin)
    See all IGFBP3 primary antibodies
  • Description
    Rabbit polyclonal to IGFBP3 (Biotin)
  • ConjugationBiotin
  • Specificityab84228 allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IGFBP3 in ELISA assays and 1.5 - 3.0 ng/lane in Western blot, under either reducing or non-reducing conditions.
  • Tested applicationsSuitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human IGFBP3

  • Positive control
    • Recombinant human IGFBP3.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 32 kDa.
ELISA Use a concentration of 0.25 - 1 µg/ml.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGFBP3 (ab109790).

Target

  • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Tissue specificityExpressed by most tissues. Present in plasma.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Developmental stageIGFBP3 levels are higher during extrauterine life and peak during puberty.
  • DomainThe thyroglobulin type-1 domain mediates interaction with HN.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acid stable subunit of the 140 K IGF complex antibody
    • Binding protein 29 antibody
    • Binding protein 53 antibody
    • BP 53 antibody
    • BP53 antibody
    • Growth hormone dependent binding protein antibody
    • IBP 3 antibody
    • IBP-3 antibody
    • IBP3 antibody
    • IBP3_HUMAN antibody
    • IGF binding protein 3 antibody
    • IGF-binding protein 3 antibody
    • IGFBP 3 antibody
    • IGFBP-3 antibody
    • IGFBP3 antibody
    • Insulin Like Growth Factor Binding Protein 3 antibody
    • Insulin-like growth factor binding protein 3 precursor antibody
    • Insulin-like growth factor-binding protein 3 antibody
    see all

References for Anti-IGFBP3 antibody (Biotin) (ab84228)

ab84228 has not yet been referenced specifically in any publications.

Product Wall

Thank you for that clarification and sorry for the delay in my reply. The whole of the Scientific Support team were out for training yesterday so we are just catching up on enquiries now.

I have had a look through the experimental details you...

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Thank you for getting back to me with your progress. I am sorry to hear the results are not as hoped for yet.

Thank you for providing the details of your experiment. I would like to clarify a few points to make sure I understand the experimen...

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Thank you for your reply.

I would like to reassure you that ab83137 Anti-IGF1 antibody (Biotin) and ab9572 Anti-IGF1 antibody are tested and covered by our 6 month guarantee for sandwich ELISA together, and human samples. In the event that a...

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Thank you for your reply.

I can confirm that ab84228 can be paired for Sandwich ELISA with Rabbit polyclonal to IGFBP3 (ab109790). I can suggest touse the polyclonal ab84228 as the detection antibody as this is the conjugated one (biotin con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"