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Rabbit polyclonal to IGFBP3 - N-terminal
Predicted to work with:
KLH-conjugated synthetic peptide selected from the N-terminal region of Rat IGFBP3
WB: HepG2 cell line lysate;
IHC: Human hepatocarcinoma tissue .
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 32 kDa.
1/10 - 1/50.
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Expressed by most tissues. Present in plasma.
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
IGFBP3 levels are higher during extrauterine life and peak during puberty.
The thyroglobulin type-1 domain mediates interaction with HN.
Phosphorylation sites are present in the extracelllular medium.
Information by UniProt
Acid stable subunit of the 140 K IGF complex antibody
Binding protein 29 antibody
Binding protein 53 antibody
Western blot - Anti-IGFBP3 antibody - N-terminal (ab75988)
Anti-IGFBP3 antibody - N-terminal (ab75988) at 1/60 dilution + HepG2 cell line lysates at 35 µg
Predicted band size: 32 kDa Observed band size: 32 kDa Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP3 antibody - N-terminal (ab75988)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue with 1/10-1/50 ab75988, used in conjunction with a peroxidase-conjugated secondary antibody, followed by DAB staining.
has not yet been referenced specifically in any publications.
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