Overview

  • Product nameAnti-IGFBP4 antibody
    See all IGFBP4 primary antibodies
  • Description
    Goat polyclonal to IGFBP4
  • SpecificityBased on ELISA and immunoblotting, this antibody shows less than 1 % cross-reactivity with recombinant human IGFBP1 and no cross reactivity with recombinant human IGFBP2 and recombinant human IGFBP3.
  • Tested applicationsSuitable for: ELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab10734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Neutralising
  • Application notesELISA: Use at a concentration of 0.5 - 1.0 µg/ml. The detection limit for recombinant human IGFBP4 is approximately 0.06 ng/well.
    Neut: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detects bands of approximately 25 and 32 kDa. The detection limit for recombinant human IGFBP4 is approximately 2 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively.

    The Neutralization Dose50 (ND50) for anti human IGFBP4 is approximately 10 to 30 µg/ml in the presence of 0.3 µg/ml of recombinant human IGFBP4 and 14 ng/ml of recombinant human IGF-II using the human breast cancer cell line MCF-7 cells. The ND50 is the concentration of antibody required to yield one half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response. The exact concentration of antibody required to neutralize human IGFBP4 activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Sequence similaritiesContains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI875747 antibody
      • BP 4 antibody
      • BP4 antibody
      • Deb2 antibody
      • HT29 IGFBP antibody
      • IBP 4 antibody
      • IBP-4 antibody
      • IBP4 antibody
      • IBP4_HUMAN antibody
      • IGF binding protein 4 antibody
      • IGF-binding protein 4 antibody
      • IGF-BP4 antibody
      • IGFBP 4 antibody
      • IGFBP-4 antibody
      • IGFBP4 antibody
      • Insulin Like Growth Factor Binding Protein 4 antibody
      • Insulin-like growth factor-binding protein 4 antibody
      see all

    References for Anti-IGFBP4 antibody (ab10734)

    ab10734 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"