Overview

  • Product name
  • Description
    Rabbit polyclonal to IGFBP4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Goat, Horse, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human IGFBP4 aa 200 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P22692

  • Positive control
    • This antibody gave a positive signal in Human, Mouse and Rat Heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab153654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).

Target

Images

  • All lanes : Anti-IGFBP4 antibody (ab153654) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 16 kDa (possible non-specific binding), 55 kDa (possible non-specific binding)


    Exposure time: 8 minutes


    IGFBP4 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab153654 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:
  • Mikkelsen JH  et al. Indirect targeting of IGF receptor signaling in vivo by substrate-selective inhibition of PAPP-A proteolytic activity. Oncotarget 5:1014-25 (2014). Read more (PubMed: 24572990) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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