Overview

  • Product nameAnti-IGFBP4 antibody
    See all IGFBP4 primary antibodies
  • Description
    Rabbit polyclonal to IGFBP4
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (RALERLAASQ SRTHEDLYII PIPNCDRNGN FHPKQCHPAL DGQRGKCWCV) of human IGFBP4 (NP_001543).

  • Positive control
    • Fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23660500
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

Anti-IGFBP4 antibody images

  • Anti-IGFBP4 antibody (ab83846) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • ab83846 at 5 µg/ml staining IGFBP1 in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab83846 at 5 µg/ml staining IGFBP1 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • ab83846 at 5 µg/ml staining IGFBP1 in Human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of mouse small intestine tissue, staining IGFBP4 with ab83846.

    Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 8). Samples were incubated with primary antibody (1/200 in PBS-T) for 12 hours at 4°C. An AlexaFluor®555-conjugated donkey anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-IGFBP4 antibody (ab83846)

This product has been referenced in:
  • Shi Z  et al. SOX9 directly regulates IGFBP-4 in the intestinal epithelium. Am J Physiol Gastrointest Liver Physiol 305:G74-83 (2013). IHC-P, ICC/IF . Read more (PubMed: 23660500) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (heart)
Permeabilization Yes - 0.3% Triton X
Specification heart
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Suwannee Thet

Verified customer

Submitted Jun 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (small intestine)
Specification small intestine
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE (PH8.0)
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 28 2012

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