Recombinant Anti-IGFBP4 antibody [EPR19762] (ab205581)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19762] to IGFBP4
- Suitable for: WB, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IGFBP4 antibody [EPR19762]
See all IGFBP4 primary antibodies -
Description
Rabbit monoclonal [EPR19762] to IGFBP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549 and HT-29 whole cell lysates; Human placenta lysate. Flow Cyt (intra): A549 cells. IP: A549 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19762 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 28, 18 kDa (predicted molecular weight: 28 kDa).
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IP |
1/30.
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Flow Cyt (Intra) |
1/500.
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Notes |
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WB
1/1000. Detects a band of approximately 28, 18 kDa (predicted molecular weight: 28 kDa). |
IP
1/30. |
Flow Cyt (Intra)
1/500. |
Target
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3487 Human
- Omim: 146733 Human
- SwissProt: P22692 Human
- Unigene: 462998 Human
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Alternative names
- AI875747 antibody
- BP 4 antibody
- BP4 antibody
see all
Images
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Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 18,28 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.
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Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + Human placenta tissue lysate at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling IGFBP4 with ab205581 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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IGFBP4 was immunoprecipitated from 0.35 mg of A549 (human lung carcinoma cell line) whole cell lysate with ab205581 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205581 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: A549 whole cell lysate 10 µg (Input).
Lane 2: ab205581 IP in A549 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205581 in A549 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Note: Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab205581 has not yet been referenced specifically in any publications.