Anti-IGFBP5 antibody (Biotin) (ab84229)

Overview

  • Product nameAnti-IGFBP5 antibody (Biotin)
    See all IGFBP5 primary antibodies
  • Description
    Rabbit polyclonal to IGFBP5 (Biotin)
  • ConjugationBiotin
  • Specificityab84229 allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IGFBP5 in ELISA assays and 1.5 - 3.0 ng/lane in Western blot, under either reducing or non-reducing conditions.
  • Tested applicationsSuitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human IGFBP5.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab84229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Sandwich ELISA
  • Application notesELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well).
    sELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well).
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Tissue specificityOsteosarcoma, and at lower levels in liver, kidney and brain.
    • Sequence similaritiesContains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • IBP 5 antibody
      • IBP-5 antibody
      • IBP5 antibody
      • IBP5_HUMAN antibody
      • IGF binding protein 5 antibody
      • IGF BP5 antibody
      • IGF-binding protein 5 antibody
      • IGFBP 5 antibody
      • IGFBP-5 antibody
      • IGFBP5 antibody
      • Insulin like growth factor binding protein 5 antibody
      • Insulin-like growth factor-binding protein 5 antibody
      see all

    Anti-IGFBP5 antibody (Biotin) images

    • Anti-IGFBP5 antibody (Biotin) (ab84229) at 0.1 µg/ml + Recombinant Human IGFBP5 protein (ab49835) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for Anti-IGFBP5 antibody (Biotin) (ab84229)

    ab84229 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"