Recombinant
RabMAb

Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Overview

  • Product name
    Anti-IGFBP7 antibody [EPR11912(B)]
    See all IGFBP7 primary antibodies
  • Description
    Rabbit monoclonal [EPR11912(B)] to IGFBP7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IGFBP7 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16270

  • Positive control
    • SW480, 293T and MCF7 cell lysates; Human pancreas, prostate and lung cancer tissues; MCF7 cells; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P 1/250.

See IHC antigen retrieval protocols.

ICC/IF 1/100.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGM antibody
    • Angiomodulin antibody
    • FSTL2 antibody
    • IBP-7 antibody
    • IBP7 antibody
    • IBP7_HUMAN antibody
    • IGF binding protein 7 antibody
    • IGF-binding protein 7 antibody
    • IGFBP rP1 antibody
    • IGFBP-7 antibody
    • IGFBP-rP1 antibody
    • IGFBP7 antibody
    • IGFBPRP1 antibody
    • Insulin like growth factor binding protein 7 antibody
    • Insulin-like growth factor-binding protein 7 antibody
    • MAC25 antibody
    • MAC25 protein antibody
    • PGI2 stimulating factor antibody
    • PGI2-stimulating factor antibody
    • Prostacyclin stimulating factor antibody
    • Prostacyclin-stimulating factor antibody
    • PSF antibody
    • RAMSVPS antibody
    • TAF antibody
    • Tumor-derived adhesion factor antibody
    see all

Images

  • All lanes : Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/1000 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa



    This image was produced using unpurified antibody.

  • Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with Purified ab171085 at 1:100 (6.0 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with purified ab171085 at 1:60 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/10000 dilution (Purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IGFBP7 with purified ab171085 at 1:250 dilution (2.4 ?g/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Flow cytometric analysis of permeabilized 293T cells labeling IGFBP7 with ab171085 at 1/250 dilution (red) compared to a rabbit IgG negative control (green). This image was produced using unpurified antibody.

  • Immunofluorescent analysis of MCF7 cells labeling IGFBP7 with ab171085 at 1/250 dilution. This image was produced using unpurified antibody.

  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.

References

This product has been referenced in:
  • Sill M  et al. Protein Profiling Gastric Cancer and Neighboring Control Tissues Using High-Content Antibody Microarrays. Microarrays (Basel) 5:N/A (2016). IHC . Read more (PubMed: 27600085) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171085.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up