Anti-IgG antibody [EM-07] (ab77118)

Overview

  • Product name
    Anti-IgG antibody [EM-07]
    See all IgG primary antibodies
  • Description
    Mouse monoclonal [EM-07] to IgG
  • Specificity
    Reacts with Fc part of human IgG heavy chain and with isolated Fc fragments. It does not cross-react with IgM.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein of Human IgG Fc fragment

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purity >95% by SDS-PAGE.
  • Clonality
    Monoclonal
  • Clone number
    EM-07
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 150 kDa (IgG).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References for Anti-IgG antibody [EM-07] (ab77118)

    ab77118 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Sample
    Human Tissue sections (Human lung tissue)
    Specification
    Human lung tissue
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 20 2014

    Thank you for contacting us.

    Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, ...

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    Thank you for your enquiry.

    I can confirm that ab77118 is suitable for detection of human IgG in ELISA. It is tested in human only and is not tested on rabbit.

    I am assuming the customer is wanting to detect rabbit IgG in their sa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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