Overview

  • Product name
  • Description
    Rabbit polyclonal to IgG1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IgG1 aa 81-110 (intracellular) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P01857

  • Positive control
    • HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab170461 is purified through a protein A column, followed by peptide affinity purification
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.

Target

  • Relevance
    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • Cellular localization
    Secreted. Cell membrane; Single-pass membrane protein
  • Database links
  • Alternative names
    • Ig gamma 1 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG1 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    see all

Images

  • All lanes : Anti-IgG1 antibody (ab170461) at 1/2000 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Human fetal thymus lysate
    Lane 3 : Human spleen lysate
    Lane 4 : Human plasma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG

    Predicted band size: 36 kDa

  • Flow cytometry analysis of HepG2 cells labeling IgG1 (green, right histogram), using ab170461 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.

References

ab170461 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab170461.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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