Recombinant
RabMAb

Anti-IgG1 antibody [EPR4417] (ab108969)

Overview

  • Product name
    Anti-IgG1 antibody [EPR4417]
    See all IgG1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4417] to IgG1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human IgG1 (Hinge).

  • Positive control
    • Human tonsil and human spleen lysates.
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 3.87 x 10 -10 M Learn more about KD
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR4417
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.

Target

  • Relevance
    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • Cellular localization
    Secreted. Cell membrane; Single-pass membrane protein
  • Database links
  • Alternative names
    • Ig gamma 1 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG1 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    see all

Anti-IgG1 antibody [EPR4417] images

  • All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)

    Lane 1 : Human tonsil lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 36 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/1000 dilution (unpurified)

    Lane 1 : IgG1 at 0.01 µg
    Lane 2 : IgG2 at 0.01 µg
    Lane 3 : IgG3 at 0.01 µg
    Lane 4 : IgG4 at 0.01 µg
    Lane 5 : Human tonsil lysate at 10 µg
    Lane 6 : Human spleen lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 36 kDa
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-IgG1 antibody [EPR4417] (ab108969)

ab108969 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab108969.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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