Anti-IgG2 antibody [52G1] (ab1933)

Overview

  • Product nameAnti-IgG2 antibody [52G1]
    See all IgG2 primary antibodies
  • Description
    Mouse monoclonal [52G1] to IgG2
  • SpecificitySpecific to -2 epitope inside Fc fragment of human IgG2. Reacts with fc region. No cross-reaction with human IgG1, IgG3, IgG4, IgA, IgM and IgE.
  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human IgG2 from myeloma patient serum.

  • Epitopenot mapped
  • Positive control
    • recombinant protein (calbiochem)
  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurity tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone number52G1
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notessELISA: Use at an assay dependent dilution as capture antibody.
    Can be paired for ELISA with Mouse monoclonal [5A9] to Human IgG (HRP) (ab7499).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • Ig gamma-2 chain C region antibody
      • IGHG2 antibody
      • immunoglobulin Gm2 antibody
      • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
      • immunoglobulin heavy constant, gamma 2 antibody
      see all

    References for Anti-IgG2 antibody [52G1] (ab1933)

    ab1933 has not yet been referenced specifically in any publications.

    Product Wall

    I have reviewed these products and have been in touch with the labs regarding reactivity with Rhesus as requested. We would not recommend any human IgG2 antibody which we know is directed to the hinge region, such as ab1935, against Rhesus as the homol...

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    I can confirm that we have tested the antibodies ab7499 (Mouse monoclonal [5A9] to Human IgG (HRP) and ab1933 (Mouse monoclonal [52G1] to Human IgG2 (ab1933) in Sandwich ELISA. This was using ab1933 as capture and HRP-conjugated ab7499 for detection...

    Read More

    The concentration of this lot is 10.7 mg/ml. My apologies for the delay.Good luck with your experiments.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"