Overview

  • Product nameAnti-IgG2a antibody [10D7A7B2]
    See all IgG2a primary antibodies
  • Description
    Mouse monoclonal [10D7A7B2] to IgG2a
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified) corresponding to IgG2a.

  • Positive control
    • Yeast membranes
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab113683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC/IF Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended.

Target

  • RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens.
  • Cellular localizationSecreted

Anti-IgG2a antibody [10D7A7B2] images

  • All lanes : Anti-IgG2a antibody [10D7A7B2] (ab113683)

    Lane 1 : Yeast whole cell lysate
    Lane 2 : yeast membrane preparation

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 95 kDa

References for Anti-IgG2a antibody [10D7A7B2] (ab113683)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"