Overview

  • Product name
    Anti-IgG2b antibody [KT93]
  • Description
    Rat monoclonal [KT93] to IgG2b
  • Host species
    Rat
  • Specificity
    ab167476 does not cross-react with mouse IgG1, IgG2a and IgM. It has weak cross-reaction with mouse IgG3.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse IgG2b.

  • Positive control
    • Mouse IgG2b.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    KT93
  • Isotype
    IgG1

Applications

Our Abpromise guarantee covers the use of ab167476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ELISA Use a concentration of 1 µg/ml.

Target

  • Relevance
    IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists oftwo identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG apart from its IgG counter-parts. Properties of IgG2b include neutralization of toxins and diffusion in the extracellular space.
  • Cellular localization
    Isoform 1: Cell membrane; Single-pass membrane protein Potential. Isoform 2: Secreted
  • Database links
  • Alternative names
    • Gamma2b antibody
    • IgG2b antibody
    • Igh 3 antibody
    • Immunoglobulin heavy constant gamma 2B antibody

Images

  • All lanes : Anti-IgG2b antibody [KT93] (ab167476) at 1 µg/ml

    Lane 1 : Mouse IgG
    Lane 2 : Mouse IgG3
    Lane 3 : Mouse IgG2a
    Lane 4 : Mouse IgG1
    Lane 5 : Mouse IgG2b

    Predicted band size: 44 kDa

References

ab167476 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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