Anti-IGLC2 antibody [5E12B9] (ab233563)
Key features and details
- Mouse monoclonal [5E12B9] to IGLC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-IGLC2 antibody [5E12B9] -
Description
Mouse monoclonal [5E12B9] to IGLC2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IGLC2 aa 1 to the C-terminus. Expressed in E. coli.
Database link: P01842 -
Positive control
- WB: Recombinant human IGLC2 protein (amino acids 1-106). IGLC2 (amino acids: 1-106) - hIgGFc transfected HEK-293 cell lysate. IHC-P: Human colon tissue and endometrial cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
5E12B9 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 11 kDa.
|
|
IHC-P |
1/200 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 11 kDa. |
IHC-P
1/200 - 1/1000. |
Target
-
Database links
- Entrez Gene: 3538 Human
- SwissProt: P01842 Human
-
Alternative names
- Ig lambda chain C regions antibody
- IGLC antibody
- immunoglobulin lambda constant region 2 (Kern- Oz- marker) antibody
see all
Images
-
All lanes : Anti-IGLC2 antibody [5E12B9] (ab233563) at 1/500 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : IGLC2 (amino acids: 1-106) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 11 kDa -
Paraffin-embedded human endometrial cancer tissue stained for IGLC2 with ab233563 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human colon tissue stained for IGLC2 with ab233563 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
-
Anti-IGLC2 antibody [5E12B9] (ab233563) at 1/500 dilution + Recombinant human IGLC2 protein (amino acids 1-106)
Predicted band size: 11 kDa(Expected MW is 36.8 kDa)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab233563 has been referenced in 1 publication.
- Guo W et al. Integrating microarray-based spatial transcriptomics and single-cell RNA-sequencing reveals tissue architecture in esophageal squamous cell carcinoma. EBioMedicine 84:104281 (2022). PubMed: 36162205