Overview

  • Product name
    Anti-IGLON5 antibody
  • Description
    Rabbit polyclonal to IGLON5
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PTITDVTSAR TALGRAALLC CEAMAVPPAD FQWYKDDRLL SSGTAEGLKV QTERTRSMLL FANVSARHYG NY, corresponding to amino acids 218-289 of Human IGLON5.

  • Positive control
    • Human nasopharynx tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Belongs to the immunoglobulin superfamily. IgLON family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • hCG1651476 antibody
    • Ig like domain containing protein ENSP00000270642 antibody
    • IGLO5_HUMAN antibody
    • IgLON family member 5 antibody
    • Iglon5 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122763 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122763, at 1/50 dilution, staining IGLON5 in paraffin-embedded Human nasopharynx tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Sabater L  et al. Cellular investigations with human antibodies associated with the anti-IgLON5 syndrome. J Neuroinflammation 13:226 (2016). Human . Read more (PubMed: 27586161) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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