Overview

  • Product name
    Anti-IgM antibody [KT103] (HRP)
    See all IgM primary antibodies
  • Description
    Mouse monoclonal [KT103] to IgM (HRP)
  • Host species
    Mouse
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat
    Does not react with: Mouse, Rabbit, Human
  • Immunogen

    Full length protein corresponding to Rat IgM.

  • Positive control
    • Rat IgM

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab170327 is purified through a Protein A column and labeled with horseradish peroxidase.
  • Clonality
    Monoclonal
  • Clone number
    KT103
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000.
WB 1/1000.

Dilution using DAB as substrate. More dilution is needed for ECL system.

Target

  • Relevance
    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
  • Cellular localization
    Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.
  • Database links
  • Alternative names
    • AGM1 antibody
    • Constant region of heavy chain of IgM antibody
    • DKFZp686I15196 antibody
    • DKFZp686I15212 antibody
    • FLJ00385 antibody
    • Hepatitis B virus receptor binding protein antibody
    • Ig mu chain C region antibody
    • IGHM antibody
    • IgM heavy chain constant region antibody
    • Immunoglobulin heavy constant mu antibody
    • Immunoglobulin mu chain antibody
    • Imunoglobulin heavy chain antibody
    • Imunoglobulin heavy chain constant region mu antibody
    • Imunoglobulin heavy chain mu constant region antibody
    • MGC104996 antibody
    • MGC52291 antibody
    • MU antibody
    • VH antibody
    see all

Images

  • All lanes : Anti-IgM antibody [KT103] (HRP) (ab170327) at 1/1000 dilution

    Lane 1 : rat IgG1
    Lane 2 : rat IgG2a
    Lane 3 : rat IgG2b
    Lane 4 : rat IgG2c
    Lane 5 : rat IgM

    Performed under reducing conditions.
  • ELISA for crossreactivity of ab170327 to various immunoglobulins.

References

ab170327 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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