Overview

  • Product name
    Anti-IGSF1 antibody
  • Description
    Rabbit polyclonal to IGSF1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: CNVNILCHGW LQDLVFMLFK EGYAEPVDYQ VPTGTMAIFS IDNLTPEDEG , corresponding to amino acids 146-195 of Human IGSF1

  • Positive control
    • WB: HepG2 cell lysate IHC-P: Human kidney tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 148 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    IGSF1 is a member of the immunoglobulin (Ig) superfamily. They have a variety of functions, but all appear to play a role in cell recognition and the regulation of cell behavior.
  • Cellular localization
    Isoforms 1 and 2: Membrane; Multipass membrane protein (Potential). Isoform 3: Secreted (Potential).
  • Database links
  • Alternative names
    • IGCD1 antibody
    • IGDC1 antibody
    • Immunoglobulin like domain containing protein 1 antibody
    • Immunoglobulin superfamily member 1 antibody
    • InhBP antibody
    • Inhibin binding protein antibody
    • KIAA0364 antibody
    • MGC75490 antibody
    • p120 antibody
    • PGSF2 antibody
    • Pituitary gland specific factor 2 antibody
    see all

Images

  • Anti-IGSF1 antibody (ab66509) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 148 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IGSF1 with ab66509 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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