Recombinant
RabMAb

Anti-Ihh antibody [EP1192Y] (ab52919)

Overview

  • Product name
    Anti-Ihh antibody [EP1192Y]
    See all Ihh primary antibodies
  • Description
    Rabbit monoclonal [EP1192Y] to Ihh
  • Tested applications
    Suitable for: WB, IHC-P, IHC-FoFrmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human IHH.

  • Positive control
    • Human fetal colon tissue, fetal brain lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 45 kDa.
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22558278
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
    • Tissue specificity
      Expressed in embryonic lung, and in adult kidney and liver.
    • Involvement in disease
      Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short.
      Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles.
    • Sequence similarities
      Belongs to the hedgehog family.
    • Post-translational
      modifications
      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
      Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
      Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity.
    • Cellular localization
      Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • BDA1 antibody
      • HHG-2 antibody
      • HHG2 antibody
      • IHH antibody
      • IHH_HUMAN antibody
      • Indian Hedgehog antibody
      • Indian hedgehog homolog antibody
      • Indian hedgehog protein antibody
      • Indian hedgehog protein C-product antibody
      • Indian hedgehog protein N-product antibody
      see all

    Anti-Ihh antibody [EP1192Y] images

    • Anti-Ihh antibody [EP1192Y] (ab52919) at 1/10000 dilution + Human fetal brain lysate at 10 µg

      Secondary
      Goat anti rabbit hrp conjugated at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa
    • Ab52919 at 1/250 dilution staining human fetal colon tissue; paraffin embedded.
    • Immunohistochemical analysis of murine intervertebral discs, staining Ihh (red) with ab52919.

      Sections were fixed in paraformaldehyde, permeabilized using 0.2% Triton X-100 for 20 minutes and blocked in blocking buffer for one hour. Samples were incubated with diluted primary antibody overnight at 4°C before being incubated with a Cy5®-conjugated goat anti-rabbit IgG as secondary antibody.

    References for Anti-Ihh antibody [EP1192Y] (ab52919)

    This product has been referenced in:
    • Wang W  et al. Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate. PLoS Genet 12:e1006352 (2016). IHC ; Mouse . Read more (PubMed: 27741240) »
    • Carbone A  et al. Indian hedgehog signaling and the role of graft tension in tendon-to-bone healing: Evaluation in a rat ACL reconstruction model. J Orthop Res 34:641-9 (2016). IHC-P ; Rat . Read more (PubMed: 26447744) »

    See all 11 Publications for this product

    Product Wall

    I have tried to check the likelihood of the Anti-Ihh antibody [EP1192Y] (ab52919) being able to cross-react with the protein from bovine samples. Unfortunately, the only protein sequence I have been able to find for the bovine Ihh protein is of the Uni...

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    Ich habe Ihre Ergebnisse an das Labor, das diesen Antikoerper herstellt, weitergeleitet.
    Es wird nun nachgeforscht, wo das Problem liegt.
    Da ich nicht moechte,dass Sie so lange warten muessen, wuerde ich Ihnen gernen einen alternativen Antiko...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Chondrocytes)
    Specification
    Chondrocytes
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
    Username

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    Verified customer

    Submitted Jan 27 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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