The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000. Predicted molecular weight: 45 kDa.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 22558278
Is unsuitable for Flow Cyt or IP.
Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
Expressed in embryonic lung, and in adult kidney and liver.
Involvement in disease
Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short. Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles.
Belongs to the hedgehog family.
The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity. Cholesterylation is required for N-product targeting to lipid rafts and multimerization. Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity.
Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
Ab52919 at 1/250 dilution staining human fetal colon tissue; paraffin embedded.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y] (ab52919)Image from Dahia CL et al., PLoS One. 2012;7(4):e35944. Epub 2012 Apr 27. Fig 5.; doi:10.1371/journal.pone.0035944; April 27, 2012, PLoS ONE 7(4): e35944.
Immunohistochemical analysis of murine intervertebral discs, staining Ihh (red) with ab52919.
Sections were fixed in paraformaldehyde, permeabilized using 0.2% Triton X-100 for 20 minutes and blocked in blocking buffer for one hour. Samples were incubated with diluted primary antibody overnight at 4°C before being incubated with a Cy5®-conjugated goat anti-rabbit IgG as secondary antibody.
This product has been referenced in:
Bao J et al. Wnt/ß-catenin and Hedgehog pathways are involved in the inflammatory effect of Interleukin 18 on rat chondrocytes. Oncotarget8:109962-109972 (2017).
Read more (PubMed: 29299122) »
Carbone A et al. Indian hedgehog signaling and the role of graft tension in tendon-to-bone healing: Evaluation in a rat ACL reconstruction model. J Orthop Res34:641-9 (2016).
Read more (PubMed: 26447744) »