Overview

  • Product nameAnti-IHPK3 antibody
    See all IHPK3 primary antibodies
  • Description
    Rabbit polyclonal to IHPK3
  • Specificityab65035 detects endogenous levels of total IHPK3 protein. Will cross-react with IHPK1 and IHPK2 which appear as the two higher MW bands in WB.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human IHPK3

  • Positive control
    • IHC-P: Human brain tissue. WB: 293 cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab65035 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).
ELISA 1/20000.

Target

Anti-IHPK3 antibody images

  • ab65035, at a 1/50 dilution, staining IHPK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
  • All lanes : Anti-IHPK3 antibody (ab65035) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-IHPK3 antibody (ab65035)

ab65035 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65035.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"