Overview

  • Product name
  • Description
    Rabbit polyclonal to IHPK3
  • Specificity
    ab65035 detects endogenous levels of total IHPK3 protein. Will cross-react with IHPK1 and IHPK2 which appear as the two higher MW bands in WB.
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human IHPK3

  • Positive control
    • IHC-P: Human brain tissue. WB: 293 cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab65035 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).
ELISA 1/20000.

Target

Anti-IHPK3 antibody images

  • ab65035, at a 1/50 dilution, staining IHPK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
  • All lanes : Anti-IHPK3 antibody (ab65035) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-IHPK3 antibody (ab65035)

ab65035 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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