Overview

  • Product nameAnti-IKAP antibody
    See all IKAP primary antibodies
  • Description
    Rabbit polyclonal to IKAP
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Synthetic peptide:

    IHQDDFGESKFITV

    , corresponding to amino acids 148-161 of Human IKAP.

  • Positive control
    • Human lung, nerve tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 150 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
    Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
  • Involvement in diseaseDefects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
  • Sequence similaritiesBelongs to the ELP1/IKA1 family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781H1425 antibody
    • DYS antibody
    • Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
    • Elongator complex protein 1 antibody
    • ELP 1 antibody
    • ELP1 antibody
    • ELP1_HUMAN antibody
    • FD antibody
    • FLJ12497 antibody
    • IKAP antibody
    • IkappaB kinase complex associated protein antibody
    • IkappaB kinase complex-associated protein antibody
    • ikbkap antibody
    • IKI 3 antibody
    • IKI3 antibody
    • IKK complex associated protein antibody
    • IKK complex-associated protein antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody
    • OTTHUMP00000063889 antibody
    • p150 antibody
    • TOT 1 antibody
    • TOT1 antibody
    see all

Anti-IKAP antibody images

  • Staining of IKAP in Human lung, nerve tissue (formalin fixed, paraffin embedded), using ab115223 at 20 µg/ml followed by biotinylated Goat anti Rabbit IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.

References for Anti-IKAP antibody (ab115223)

ab115223 has not yet been referenced specifically in any publications.

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