Anti-IKAP antibody - Carboxyterminal end (ab62498)

Overview

  • Product nameAnti-IKAP antibody - Carboxyterminal end
    See all IKAP primary antibodies
  • Description
    Rabbit polyclonal to IKAP - Carboxyterminal end
  • SpecificityThis antibody reacts with IKAP
  • Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP.

  • Positive control
    • A20 cell lysate and A20 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148, 100 kDa (predicted molecular weight: 148 kDa).
ICC Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionMay act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
    Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
  • Involvement in diseaseDefects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
  • Sequence similaritiesBelongs to the ELP1/IKA1 family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781H1425 antibody
    • DYS antibody
    • Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
    • Elongator complex protein 1 antibody
    • ELP 1 antibody
    • ELP1 antibody
    • ELP1_HUMAN antibody
    • FD antibody
    • FLJ12497 antibody
    • IKAP antibody
    • IkappaB kinase complex associated protein antibody
    • IkappaB kinase complex-associated protein antibody
    • ikbkap antibody
    • IKI 3 antibody
    • IKI3 antibody
    • IKK complex associated protein antibody
    • IKK complex-associated protein antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody
    • OTTHUMP00000063889 antibody
    • p150 antibody
    • TOT 1 antibody
    • TOT1 antibody
    see all

Anti-IKAP antibody - Carboxyterminal end images

  • Lane 1 : Anti-IKAP antibody - Carboxyterminal end (ab62498) at 0.5 µg/ml
    Lane 2 : Anti-IKAP antibody - Carboxyterminal end (ab62498) at 1 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit IgG

    Predicted band size : 148 kDa
    Observed band size : 100,148 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry of IKAP in A20 cells with ab62498 antibody at 1 µg/ml.
  • Immunofluorescence of IKAP in A20 cells using ab62498 at 20 ug/ml.

References for Anti-IKAP antibody - Carboxyterminal end (ab62498)

ab62498 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Antigen retrieval step Heat mediated
Permeabilization No
Specification testis
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Dec 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"