Recombinant
RabMAb

Anti-IKAP antibody [EPR13113] (ab179437)

Overview

  • Product name
    Anti-IKAP antibody [EPR13113]
    See all IKAP primary antibodies
  • Description
    Rabbit monoclonal [EPR13113] to IKAP
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IKAP. The exact sequence is proprietary.
    Database link: O95163

  • Positive control
    • HepG2, HeLa, Jurkat and 293T cell lysates; HeLa cells; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab179437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 150 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
      Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
    • Involvement in disease
      Defects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
    • Sequence similarities
      Belongs to the ELP1/IKA1 family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781H1425 antibody
      • DYS antibody
      • Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
      • Elongator complex protein 1 antibody
      • ELP 1 antibody
      • ELP1 antibody
      • ELP1_HUMAN antibody
      • FD antibody
      • FLJ12497 antibody
      • IKAP antibody
      • IkappaB kinase complex associated protein antibody
      • IkappaB kinase complex-associated protein antibody
      • ikbkap antibody
      • IKI 3 antibody
      • IKI3 antibody
      • IKK complex associated protein antibody
      • IKK complex-associated protein antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody
      • OTTHUMP00000063889 antibody
      • p150 antibody
      • TOT 1 antibody
      • TOT1 antibody
      see all

    Images

    • All lanes : Anti-IKAP antibody [EPR13113] (ab179437) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 150 kDa
    • Immunofluorescent analysis of HeLa cells labeling IKAP with ab179437 at 1/50 dilution (green). DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized Jurkat cells labeling IKAP with ab179437 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab179437 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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