Overview

  • Product nameAnti-Ikaros antibody
    See all Ikaros primary antibodies
  • Description
    Goat polyclonal to Ikaros
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    DLLRAASENSQDALR

    , corresponding to amino acids 436-450 of Human Ikaros (NP_006051.1)

  • Positive control
    • MOLT4 cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
    WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).
    • Tissue specificityAbundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.
    • Involvement in diseaseDefects in IKZF1 are frequent occurrences (28.6%) in acute lymphoblasic leukemia (ALL). Such alterations or deletions lead to poor prognosis for ALL.
      Chromosomal aberrations involving IKZF1 are a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;7)(q27;p12), with BCL6.
    • Sequence similaritiesBelongs to the Ikaros C2H2-type zinc-finger protein family.
      Contains 6 C2H2-type zinc fingers.
    • DomainThe N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin.
      The C-terminal zinc-finger domain is required for dimerization.
    • Post-translational
      modifications
      Phosphorylation controls cell-cycle progression from late G(1) stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G(1) phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity). Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.
      Sumoylated. Simulataneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1.
      Polyubiquitinated.
    • Cellular localizationCytoplasm; Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events and Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity).
    • Information by UniProt
    • Database links
    • FormThere are 7 isoforms produced by alternative splicing.
    • Alternative names
      • CLL associated antigen KW 6 antibody
      • DNA-binding protein Ikaros antibody
      • hIk 1 antibody
      • Hs.54452 antibody
      • IK1 antibody
      • Ikaros (zinc finger protein) antibody
      • IKAROS antibody
      • IKAROS family zinc finger 1 (Ikaros) antibody
      • Ikaros family zinc finger protein 1 antibody
      • Ikzf1 antibody
      • IKZF1_HUMAN antibody
      • LYF1 antibody
      • Lymphoid transcription factor LyF-1 antibody
      • PRO0758 antibody
      • Zinc finger protein subfamily 1A 1 (Ikaros) antibody
      • Zinc finger protein subfamily 1A 1 antibody
      • Zinc finger protein, subfamily 1A, member 1 antibody
      • ZNFN1A1 antibody
      see all

    Anti-Ikaros antibody images

    References for Anti-Ikaros antibody (ab77411)

    ab77411 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (RS411 human pre B leukemic cell line)
    Loading amount 20 µg
    Specification RS411 human pre B leukemic cell line
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step 2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

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    Verified customer

    Submitted Jan 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"