Human Predicted to work with:
Synthetic peptide corresponding to Human IKB alpha. Synthetic non phosphopeptide derived from human IKB alpha around the phosphorylation site of tyrosine 42 (E-E-YP-E-Q
Immunogen is in the range of aa 9-58. Database link: P25963
Human breast carcinoma tissue. 293 cells extract.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
FunctionInhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Involvement in diseaseEctodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family. Contains 5 ANK repeats.
Post-translational modificationsPhosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Major histocompatibility complex enhancer-binding protein MAD3 antibody
NF kappa B inhibitor alpha antibody
NF-kappa-B inhibitor alpha antibody
Nuclear factor of kappa light chain gene enhancer in B cells antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
Anti-IKB alpha antibody images
Western blot - IKB alpha antibody (ab47424)
All lanes : Anti-IKB alpha antibody (ab47424) at 1/500 dilution
Lane 1 : extracts from 293 cells treated with TNF alpha (20ng/ml, 30min). No preincupation with immunizing peptide Lane 2 : extracts from 293 cells treated with TNF alpha (20ng/ml, 30min). Sample preincubabted with immunizing peptide.
Predicted band size : 35 kDa Observed band size : 35 kDa