• Product nameAnti-IKB alpha antibody
    See all IKB alpha primary antibodies
  • Description
    Rabbit polyclonal to IKB alpha
  • Specificityab47424 detects endogenous levels of total IKB alpha protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human IKB alpha. Synthetic non phosphopeptide derived from human IKB alpha around the phosphorylation site of tyrosine 42 (E-E-YP-E-Q Immunogen is in the range of aa 9-58.
    Database link: P25963

  • Positive control
    • Human breast carcinoma tissue. 293 cells extract.



Our Abpromise guarantee covers the use of ab47424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.


  • FunctionInhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
  • Involvement in diseaseEctodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
  • Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.
  • Post-translational
    Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B alpha antibody
    • I-kappa-B-alpha antibody
    • IkappaBalpha antibody
    • IkB-alpha antibody
    • IKBA antibody
    • IKBA_HUMAN antibody
    • IKBalpha antibody
    • MAD 3 antibody
    • MAD3 antibody
    • Major histocompatibility complex enhancer-binding protein MAD3 antibody
    • NF kappa B inhibitor alpha antibody
    • NF-kappa-B inhibitor alpha antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    see all

Anti-IKB alpha antibody images

  • All lanes : Anti-IKB alpha antibody (ab47424) at 1/500 dilution

    Lane 1 : extracts from 293 cells treated with TNF alpha (20ng/ml, 30min). No preincupation with immunizing peptide
    Lane 2 : extracts from 293 cells treated with TNF alpha (20ng/ml, 30min). Sample preincubabted with immunizing peptide.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Immunhistochemical analysis of IKB alpha expression in Paraffin embedded human breast carcinoma tissue sections using 1/50 ab47424. Left: untreated sample. Right: Immunizing peptide treated sample (negative control).

References for Anti-IKB alpha antibody (ab47424)

ab47424 has not yet been referenced specifically in any publications.

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