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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Overview
Product name
Anti-IKB alpha antibody
See all IKB alpha products (25) ...
Description
Rabbit polyclonal to IKB alpha
Tested applications
WB, IHC-FrFl, ICC, IHC-P, IHC-Fr, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length protein (Mouse).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000
IHC-FrFl: Use at an assay dependent concentration.
ICC: Use at an assay dependent concentration.
IHC-P: 1/200Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr: 1/200
ICC/IF: 1/200
IP: Use at 2 µg/mg of lysate.
Target
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Involvement in disease
Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.
Sequence similarities
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.
Post-translational
modifications
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitinatin leads to protein degredation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interefers with NFKBIA degradation and impairs subsequenbt NF-kappa-B activation.
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Target information above from: UniProt accessionP25963
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- I kappa B alpha antibody
- I(Kappa)B(alpha) antibody
- I-kappa-B-alpha antibody
see all
Database links
- Entrez Gene: 4792 Human
- Entrez Gene: 18035 Mouse
- Entrez Gene: 25493 Rat
- Omim: 164008 Human
- SwissProt: P25963 Human
- SwissProt: Q9Z1E3 Mouse
- SwissProt: Q63746 Rat
- Unigene: 81328 Human
see all
Anti-IKB alpha antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKB alpha antibody (ab7217)
ab7217 staining IKB alpha in human stomach tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody, at a 1/200 dilution, for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody.
Image courtesy of an anonymous Abreview.
Immunoprecipitation - IKB alpha antibody (ab7217)
Whole cell lysate from HeLa cells used at total protein input 200µg. Immunoprecipitation step performed using Protein A/G. Diluted at 2µg/mg lysate, 16 hours at 4°C in NETN buffer.ab7217 used as western blot antibody at a 1/1000 dilution to confirm successful Immunoprecipitation.
Image courtesy of an anonymous Abreview.
Immunohistochemistry (Frozen sections) - IKB alpha antibody (ab7217)
ab7217 staining IKB alpha in human stomach tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody.
Image courtesy of an anonymous Abreview.
Immunocytochemistry/ Immunofluorescence - IKB alpha antibody (ab7217)
ab7217 staining IKB alpha in NIH3T3 mouse fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/500 for 1 hour at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-IKB alpha antibody (ab7217)
This product has been referenced in:
- Maalouf SWet al. Inflammatory responses in epithelia: endotoxin-induced IL-6 secretion and iNOS/NO production are differentially regulated in mouse mammary epithelial cells. J Inflamm (Lond) 7:58 (2010). WB; Mouse.Read more (PubMed: 21118556) »
- Cai Bet al. Alpha7 cholinergic-agonist prevents systemic inflammation and improves survival during resuscitation. J Cell Mol Med 13:3774-85 (2009). WB; Rat.Read more (PubMed: 19602049) »
See all 3 publications for this product
Publishing research using ab7217? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"