Overview

  • Product name
  • Description
    Rabbit polyclonal to IKB alpha
  • Tested applications
    Suitable for: WB, IHC-FrFl, ICC, IHC-P, IHC-Fr, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab7217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000.
IHC-FrFl Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/200.
ICC/IF 1/200.
IP Use at 2 µg/mg of lysate.

Target

  • Function
    Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
  • Involvement in disease
    Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
  • Sequence similarities
    Belongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.
  • Post-translational
    modifications
    Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B alpha antibody
    • I-kappa-B-alpha antibody
    • IkappaBalpha antibody
    • IkB-alpha antibody
    • IKBA antibody
    • IKBA_HUMAN antibody
    • IKBalpha antibody
    • MAD 3 antibody
    • MAD3 antibody
    • Major histocompatibility complex enhancer-binding protein MAD3 antibody
    • NF kappa B inhibitor alpha antibody
    • NF-kappa-B inhibitor alpha antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    see all

Anti-IKB alpha antibody images

  • ab7217 staining IKB alpha in human stomach tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody, at a 1/200 dilution, for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody.

    See Abreview

  • Whole cell lysate from HeLa cells used at total protein input 200µg. Immunoprecipitation step performed using Protein A/G. Diluted at 2µg/mg lysate, 16 hours at 4°C in NETN buffer.
    ab7217 used as western blot antibody at a 1/1000 dilution to confirm successful Immunoprecipitation.

    See Abreview

  • ab7217 staining IKB alpha in human stomach tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody.

    See Abreview

  • ab7217 staining IKB alpha in NIH3T3 mouse fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/500 for 1 hour at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.

    See Abreview

References for Anti-IKB alpha antibody (ab7217)

This product has been referenced in:
  • Wei J  et al. Oxyresveratrol Is a Phytoestrogen Exerting Anti-inflammatory Effects Through NF-?B and Estrogen Receptor Signaling. Inflammation 40:1285-1296 (2017). Human . Read more (PubMed: 28484893) »
  • Liu W  et al. Electroacupuncture inhibits inflammatory injury by targeting the miR-9-mediated NF-?B signaling pathway following ischemic stroke. Mol Med Rep 13:1618-26 (2016). WB ; Rat . Read more (PubMed: 26718002) »

See all 8 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Permeabilization
Yes - 0.25%TritonX100
Fixative
Paraformaldehyde
Username

Dr. Alain Chedotal

Verified customer

Submitted Feb 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Non-reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Fabio Marongiu

Verified customer

Submitted Oct 11 2012

Codes offre de test :
ab32515 : *****
ab32096 : *****
ab7217 : *****
ab12135 : *****
ab1791 : *****

Date d’expiration : JJ MM AAAA

Je suis ravi que vous souhaitiez participer ànotre offre et t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Pancreas)
Loading amount
200000 cells
Specification
Pancreas
Treatment
With and without TNF-a
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted Dec 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain sections)
Specification
Mouse brain sections
Username

Abcam user community

Verified customer

Submitted Jan 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3 Mouse Fibroblasts)
Specification
NIH3T3 Mouse Fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (NIH3T3 Mouse Fibroblasts)
Total protein in input
200 µg
Specification
NIH3T3 Mouse Fibroblasts
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Jan 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (NIH 3T3 Mouse Fibroblasts)
Loading amount
20 µg
Specification
NIH 3T3 Mouse Fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (AGS Human gastric carcinoma cells)
Specification
AGS Human gastric carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
200 µg
Specification
HeLa cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Oct 19 2010

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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